Recombinant Mouse Trypsin 3/PRSS3 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3565-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse Trypsin 3/PRSS3 protein
Phe16-Asn246, with a C-terminal 10-His tag
Accession # NP_035775
Phe16-Asn246, with a C-terminal 10-His tag
Accession # NP_035775
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Phe16
Predicted Molecular Mass
26 kDa
SDS-PAGE
29 kDa and 36 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >17,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >17,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
3565-SE
| Formulation | Supplied as a 0.2 μm filtered solution in HCl and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Trypsin 3/PRSS3
Mouse Trypsin-3, encoded by the PRSS3 gene, is also known as mesotrypsin. It consists of a signal peptide (residues 1 to 15), a pro region (residues 16 to 23), and a mature chain (residues 24 to 246) (1). The purified recombinant mouse Trypsin 3 corresponds to the pro form, which can be activated by enterokinase.
References
- Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1483, Academic Press, San Diego.
Alternate Names
Mesotrypsin, MTG, PRSS3, PRSS4, Trypsin 4, Trypsinogen 15
Gene Symbol
PRSS3
UniProt
Additional Trypsin 3/PRSS3 Products
Product Documents for Recombinant Mouse Trypsin 3/PRSS3 Protein, CF
Product Specific Notices for Recombinant Mouse Trypsin 3/PRSS3 Protein, CF
For research use only
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