Recombinant Mouse Tryptase gamma-1/TPSG1 Protein, CF

Tryptase gamma-1, also known as transmembrane tryptase, is a serine protease synthesized as a preproenzyme with a C-terminal transmembrane anchor (1, 2). Mast cells, mediators of inflammatory and allergic response, are found throughout the body concentrated near blood vessels in connective tissue and the mucous membranes of the respiratory and gastrointestinal tract (3). Upon activation, mast cells release granules that are enriched with neutral serine proteases including tryptases, chymase and cathepsin G (4). The recombinant mouse TPSG1 was expressed as a soluble protein terminated at residue 275, lacking the transmembrane domain, and corresponded to the proenzyme. The proenzyme can be cleaved by trypsin to form the active enzyme. The serine protease activity of trypsin-activated recombinant mouse TPSG1 can be inhibited by ecotin (Catalog # http://www.rndsystems.com/product_results.aspx?k=1328-PI">1328-PI). Typically, >95% protease activity is inhibited by ecotin at approximately a 10:1 molar ratio.