Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 743-R3
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse VEGFR3/Flt-4 protein
| Met | Mouse VEGFR3 (Tyr25-Asp770) Accession # P35917 |
IEGRDID | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | |||
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
112 kDa (monomer)
SDS-PAGE
123 kDa with two additional minor bands at 73 kDa and 60 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse VEGFR3/Flt-4 Fc Chimera (Catalog # 743-R3) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF-D (Catalog # 469-VD) with an apparent Kd <50 nM.
Immobilized Recombinant Mouse VEGFR3/Flt-4 Fc Chimera (Catalog # 743-R3) at 5 μg/mL (100 μL/well) can bind Recombinant Mouse VEGF-D (Catalog # 469-VD) with an apparent Kd <50 nM.
Formulation, Preparation and Storage
743-R3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: VEGFR3/Flt-4
VEGFR3 (Flt-4), together with VEGFR1 (Flt-1) and VEGFR2 (KDR/Flk-1), belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of these receptors is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis.
In adults, VEGFR3 expression is restricted to the endothelial cells of the lymphatic vessels. Mouse VEGFR3 cDNA encodes a 1363 amino acid (aa) residue precursor protein with a 24 aa residue signal peptide. Mature VEGFR3 has a 751 aa residue extracellular domain, a 22 aa residue hydrophobic transmembrane domain and a 565 aa residue cytoplasmic domain. The polypeptide sequences of murine Flt-4 is 88% identical to the human homologue. VEGFR3 has been reported to serve as the receptors for VEGF-C and VEGF-D.
References
- Finnerty, H. et al. (1993) Oncogene 8:2293.
- Joukov, V. et al. (1996) EMBO J. 15:290.
- Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA. 95:548.
Long Name
Vascular Endothelial Growth Factor Receptor 3
Alternate Names
Flt-4, FLT4, VEGF R3
Gene Symbol
FLT4
UniProt
Additional VEGFR3/Flt-4 Products
Product Documents for Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Mouse VEGFR3/Flt-4 Fc Chimera Protein, CF
For research use only
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