Recombinant Mouse VSIG1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9645-VS

R&D Systems, part of Bio-Techne
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9645-VS-050
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Key Product Details

Source

NS0

Accession #

NP_084457

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSIG1 protein
Mouse VSIG1
(Val23-Glu234)
Accession # NP_084457		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val23

Predicted Molecular Mass

50 kDa

SDS-PAGE

63-76 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 1-6 μg/mL.

Scientific Data Images for Recombinant Mouse VSIG1 Fc Chimera Protein, CF

Recombinant Mouse VSIG1 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG1 Fc Chimera Protein Bioactivity

Recombinant Mouse VSIG1 Fc Chimera (Catalog # 9645‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC), in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.

Formulation, Preparation and Storage

9645-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG1

VSIG1 (V-set and immunoglobulin domain-containing protein 1), also known as Glycoprotein A34, is a member of the JAM (junctional adhesion molecule) family of proteins (1, 2). It is predominantly expressed in stomach and testis, and was also detected in gastric, esophageal and ovarian cancers (2). VSIG1 is required for the proper differentiation of glandular gastric epithelia, and also plays a role as tumor suppressor (3, 4). This type I transmembrane glycoprotein consists of a 22 amino acid (aa) signal peptide, a 212 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 152 aa cytoplasmic domain. Within the ECD, mouse VSIG1 shares 81% and 94% amino acid sequence identity with human and rat VSIG1, respectively. VSIG1 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG1 inhibits T cell activation, including IL-17 and interferon gamma production.

References

  1. Kim, E. et al. (2010) Mol. Cells. 30:443.
  2. Scanlan, M.J. et al. (2006) Cancer Immun. 6:2.
  3. Oidovasambuu, O. (2011) PLoS One 10:e25908.
  4. Inoue, Y. et al. (2017) Cancer Sci. 8:1701.

Long Name

V-Set and Immunoglobulin Domain Containing 1

Alternate Names

GPA34

Entrez Gene IDs

340547 (Human); 78789 (Mouse); 315920 (Rat); 102123142 (Cynomolgus Monkey)

Gene Symbol

VSIG1

UniProt

NP_084457

Additional VSIG1 Products

Product Documents for Recombinant Mouse VSIG1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse VSIG1 Fc Chimera Protein, CF

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