Recombinant Mouse VSIG4 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9747-VS

R&D Systems, part of Bio-Techne
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9747-VS-050
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Key Product Details

Source

NS0

Accession #

NP_808457

Conjugate

Unconjugated

Applications

Bioactivity

View all VSIG4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse VSIG4 protein
His20-Pro187, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His20

Predicted Molecular Mass

20 kDa

SDS-PAGE

29-38 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 0.5-2.5 μg/mL.

Scientific Data Images for Recombinant Mouse VSIG4 His-tag Protein, CF

Recombinant Mouse VSIG4 His-tag Protein Bioactivity

Recombinant Mouse VSIG4 His-tag Protein Bioactivity

Recombinant Mouse VSIG4 (Catalog # 9747-VS) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.5-2.5 μg/mL.

Formulation, Preparation and Storage

9747-VS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: VSIG4

VSIG4 (Vset and immunoglobulin domain containing 4), also known as complement receptor immunoglobulin (CRIg) and Z39IG, is a 45 kDa, type I transmembrane protein of the B7 family within the Ig superfamily that is expressed only in tissue resident macrophages (1-4). The gene is located on the X chromosome (2). Mouse VSIG4 is synthesized as a 280 amino acid (aa) precursor that contains a signal sequence, an IgV-type immunological domain, one potential N-linked glycosylation site, and a single transmembrane domain (4). The IgV domain of mouse VSIG4 shares 86% and 80% aa sequence identity with the IgV domains of rat and human VSIG4, respectively. VSIG4 is specifically expressed on macrophages in the thymic medulla, peritoneum, alveoli, synovia, adipose and heart, liver Kupffer cells, placental Hofbauer cells, and atherosclerotic foam cells (1-4, 6-9). It is absent on infiltrating macrophages (8). VSIG4 is a complement receptor that binds C3b and iC3b fragments, internalizes them to recycling endosomes, and is recycled to the cell surface (4, 6). It contributes significantly to innate immunity by binding and phagocytosis of complement opsonized invading pathogens (4, 8, 10). Binding of either native or recombinant soluble VSIG4 to C3b inhibits complement amplification through the alternative, but not classical, pathway (10, 11). VSIG4 is also a negative regulator of mouse and human T cell activation (2). Although VSIG4 engagement may activate NF kappaB and thus be proinflammatory in some cases, many of its activities are important in resolving, rather than initiating, inflammation (1, 2, 7, 10, 11). There is emerging evidence in human conditions that VSIG-4 may be a valuable biomarker in infection and immunity, inflammatory conditions and cancer prognosis (12-14).

References

  1. He, J.Q. et al. (2008) Mol. Immunol. 45:4041.
  2. Vogt, L. et al. (2006) J. Clin. Invest. 116:2817.
  3. Langnaese, K. et al. (2000) Biochim. Biophys. Acta 1492:522.
  4. Helmy, K.Y. et al. (2006) Cell 124:915.
  5. Entrez protein Accession # EAX05393, NP_001093901, CAI42052, CAI4205, EAX05394.
  6. Tanaka, M. et al. (2008) Clin. Exp. Immunol. 154:38.
  7. Lee, M.Y . et al. (2006) J. Leukoc. Biol. 80:922.
  8. Gorgani, N.N. et al. (2008) J. Immunol. 181:7902.
  9. Walker, M.G. (2002) Biochim. Biophys. Acta 1574:387.
  10. Wiesmann, C. et al. (2006) Nature 444:217.
  11. Katschke, K.J. et al. (2007) J. Exp. Med. 204:1319.
  12. Small, A.G. et al. (2016) Swiss Med. Wkly. 146:w14301.
  13. Roh J., et al. (2017) Oncotarget 8:58122.
  14. Kim K.H., et al. (2016) Autophagy 12:1647.

Long Name

V-Set and Immunoglobulin Domain Containing 4

Alternate Names

Z39IG

Entrez Gene IDs

11326 (Human); 278180 (Mouse); 312102 (Rat); 102144784 (Cynomolgus Monkey)

Gene Symbol

VSIG4

UniProt

NP_808457

Additional VSIG4 Products

Product Documents for Recombinant Mouse VSIG4 His-tag Protein, CF

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Product Specific Notices for Recombinant Mouse VSIG4 His-tag Protein, CF

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