Recombinant Mouse Wnt-1/sFRP-1 Complex Protein

R&D Systems, part of Bio-Techne | Catalog # 9765-WN

R&D Systems, part of Bio-Techne
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9765-WN-010
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Key Product Details

Source

CHO

Accession #

P04426, Q8C4U3

Structure / Form

Non-covalent complex

Conjugate

Unconjugated

Applications

Bioactivity

View all Wnt-1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-1 protein
Mouse Wnt-1
(Ala28-Leu370)
Accession # P04426
Mouse sFRP-1
(Ser32-Lys314)
Accession # Q8C4U3
N-terminus C-terminus

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala28 (Wnt-1) & Ser32, Phe39 (sFRP-1)

Predicted Molecular Mass

38 kDa (Wnt-1) & 33 kDa (sFRP-1)

SDS-PAGE

35-59 kDa, reducing conditions

Activity

Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells.
The ED50 for this effect is 0.03-0.18 μg/mL

Scientific Data Images for Recombinant Mouse Wnt-1/sFRP-1 Complex Protein

Recombinant Mouse Wnt-1/sFRP-1 Complex Protein Bioactivity

Recombinant Mouse Wnt-1/sFRP-1 Complex Protein Bioactivity

Recombinant Mouse Wnt-1/sFRP-1 Complex (Catalog # 9765-WN) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 0.03-0.18 μg/mL.

Formulation, Preparation and Storage

9765-WN
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, NaCl, Trehalose and with BSA as a carrier protein.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Wnt-1

Wnt-1 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental processes (1). Mouse Wnt-1 cDNA encodes a 370 amino acid (aa) protein that contains four potential N-linked glycosylation sites plus 23 cysteines (2, 3). When secreted, it exists as a 343 aa glycoprotein with a molecular weight of 42 to 48 kDa. Mouse Wnt -1 shares a 99.7% aa sequence identity with human and rat Wnt-1. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta-catenin levels (4). As intracellular beta-catenin levels rise, beta-catenin binds to TCF/LEF transcription factors leading to expression of Wnt target genes (5). Wnt-1 is known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors (6,7). More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule (7). R&D Systems produces biologically active mouse Wnt-1 protein in a complex with mouse sFRP-1, and this Wnt-1/sFRP-1 complex is stable in the physiological buffer without detergent.

References

  1. Willert, K. and R. Nusse (2012) Cold Spring Harb. Perspect. Biol. 4:a007864.
  2. Van Ooyen, A. et al. (1985) EMBO J. 4:2905.
  3. Burrus, L.W. and A.P. McMahon (1995) Exp. Cell Res. 220:363.
  4. MacDonald, B.T. and X. He (2012) Cold Spring Harb. Perspect. Biol. 4:a007880.
  5. Korinek, V. et al. (1997) Science 275:1784.
  6. Dennis, S. et al. (1999) J. Cell Sci. 112:3815.
  7. Bafico, A. et al. (1999) J. Biol. Chem. 274:16180.

Long Name

Wingless-type MMTV Integration Site Family, Member 1

Alternate Names

Int-1, Wnt1

Entrez Gene IDs

7471 (Human); 22408 (Mouse)

Gene Symbol

WNT1

UniProt

P04426, Q8C4U3

Additional Wnt-1 Products

Product Documents for Recombinant Mouse Wnt-1/sFRP-1 Complex Protein

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