Recombinant Mouse ZNRF3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8328-RF

R&D Systems, part of Bio-Techne
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8328-RF-050
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Key Product Details

Source

NS0

Accession #

Q5SSZ7

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all ZNRF3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse ZNRF3 protein
Mouse ZNRF3
(Lys53-Met216)
Accession # Q5SSZ7		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys53

Predicted Molecular Mass

45 kDa

SDS-PAGE

51-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated at 0.25 µg/ml, Recombinant Mouse ZNRF3 Fc Chimera binds with an apparent Kd <0.2 nM.

Scientific Data Images for Recombinant Mouse ZNRF3 Fc Chimera Protein, CF

ZNRF3 Binds R-Spondin 3.

ZNRF3 Binds R-Spondin 3.

When Recombinant Mouse R-Spondin 3 (Catalog # 4120-RS) is coated onto a microplate well at 0.25 µg/mL, Recombinant Mouse ZNRF3 Fc Chimera (Catalog # 8328-RF) binds with an apparent Kd<0.2 nM.
ZNRF3 Binds R-Spondin 3.

ZNRF3 Binds R-Spondin 3.

1 μg/lane of Recombinant Mouse ZNRF3(Catalog # 8328-RF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 54.7 and 138 kDa, respectively.

Formulation, Preparation and Storage

8328-RF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZNRF3

ZNRF3 (zinc/RING finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).

References

  1. de Lau, W. et al. (2014) Genes Dev. 28:305.
  2. Xie, Y. et al. (2013) EMBO Rep. 14:1120.
  3. Peng, W.C. et al. (2013) PLoS One 8:e83110.
  4. Hao, H.X. et al. (2012) Nature 485:195.
  5. Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
  6. Koo, B.K. et al. (2012) Nature 488:665.
  7. Ong, C.K. et al. (2012) Nat. Genet. 44:690.
  8. Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
  9. Ryland, G.L. et al. (2013) J. Pathol. 229:469.
  10. Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.

Long Name

Zinc and Ring Finger 3

Alternate Names

BK747E2.3, KIAA1133, RNF203

Entrez Gene IDs

84133 (Human); 407821 (Mouse)

Gene Symbol

ZNRF3

UniProt

Q5SSZ7

Additional ZNRF3 Products

Product Documents for Recombinant Mouse ZNRF3 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse ZNRF3 Fc Chimera Protein, CF

For research use only

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