Recombinant P. heparinus Heparinase III Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6145-GH

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Key Product Details

Learn more about Fluorescent Glycan Labeling and Detection

Source

E. coli

Accession #

AAB18278

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Heparinase III Proteins and Enzymes »

Product Specifications

Source

E. coli-derived p. heparinus Heparinase III protein
Gln25-Pro659, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

74 kDa

SDS-PAGE

66 kDa, reducing conditions

Activity

Measured by its ability to liberate oligosaccharides from heparin.
The specific activity is >3,000 pmol/min/μg, as measured under the described conditions.

Scientific Data Images for Recombinant P. heparinus Heparinase III Protein, CF

Recombinant P. heparinus Heparinase III Protein Enzyme Activity

Recombinant P. heparinus Heparinase III Protein Enzyme Activity

Heparinase III digestion of Heparan Sulfate (200 μg) is assessed in a 5-minute kinetic assay at room temperature by monitoring absorbance at 232 nm. R&D Systems RecombinantP. heparinumHeparinase III (Catalog # 6145-GH) (orange) exhibits much greater activity than the Bacteroides Heparinase III from the competition (green).

Formulation, Preparation and Storage

6145-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Heparinase III

Heparan sulfate is a sulfated glycosaminoglycan with the repeating disaccharide units of -4HexA1,4GlcNAc beta1-. It is usually attached to the protein cores of proteoglycans found on the cell membrane and in the extracellular matrix where it binds to a variety of protein ligands and regulates a wide range of biological activities (1, 2). Heparan sulfate has a domain structure where sulfated regions are interspaced with less or non-sulfated regions (3, 4). Heparin shares the backbone structure with heparan sulfate but contains no non‑sulfated regions. Heparinases are a family of lyases that release unsaturated oligosaccharides from heparin and heparan sulfate upon digestion (5). Heparinase I recognizes highly sulfated regions and is more specific for heparin. Heparinase II digests both heparin and heparan sulfate. Heparinase III prefers less-sulfated regions and is more active on heparan sulfate (6, 7).

References

  1. MacArthur, J. M. et al. (2007) J. Clin. Invest. 117:153.
  2. Esko, J. D. and Selleck, S. B. (2002) Annu. Rev. Biochem. 71:435.
  3. Maccarana, M. et al. (1996) J. Biol. Chem. 271:17804.
  4. Linker, A. and Hovingh, P. (1975) Biochim. Biophys. Acta. 385:324.
  5. Linker, A. and Hovingh, P. (1965) J. Biol. Chem. 240:3724.
  6. Su, H. et al. (1996) Appl. Environ. Microbiol. 62:2723.
  7. Hovingh, P. and Linker, A. (1970) J. Biol. Chem. 245:6170.

Entrez Gene IDs

8254931 (P. heparinus)

Gene Symbol

PHEP_3797

UniProt

AAB18278

Additional Heparinase III Products

Product Documents for Recombinant P. heparinus Heparinase III Protein, CF

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Product Specific Notices for Recombinant P. heparinus Heparinase III Protein, CF

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