Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

R&D Systems, part of Bio-Techne | Catalog # 9188-4L

R&D Systems, part of Bio-Techne
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Carrier Free
9188-4L-025/CF
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With Carrier
9188-4L-025
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Key Product Details

Source

NS0

Accession #

Q80WE6

Conjugate

Unconjugated

Applications

Bioactivity

View all 4-1BB Ligand/TNFSF9 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat 4-1BB Ligand/TNFSF9 protein
Arg103-Gly308, with an N-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His

Predicted Molecular Mass

24 kDa

SDS-PAGE

33-43 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat 4-1BB/TNFRSF9 Fc Chimera (Catalog # 7968-4B) is Immobilized at 100 ng/mL (100 µL/well), the concentration of Recombinant Rat 4-1BB Ligand/TNFSF9 that produces 50% optimal binding response is approximately 1-6 ng/mL.

Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3.
The ED50 for for this effect is 5-30 ng/mL

Scientific Data Images for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Recombinant Rat 4-1BB Ligand/TNFSF9 Protein Bioactivity

Recombinant Rat 4-1BB Ligand/TNFSF9 Protein Bioactivity

When Recombinant Rat 4-1BB/TNFRSF9 Fc Chimera (Catalog # 7968-4B) is coated at 100 ng/mL, Recombinant Rat 4-1BB Ligand/TNFSF9 (Catalog # 9188-4L) binds with a ED50 of 1-6 ng/mL.
Recombinant Rat 4-1BB Ligand/TNFSF9 Protein Bioactivity

Recombinant Rat 4-1BB Ligand/TNFSF9 Protein Bioactivity

Recombinant Rat 4-1BB Ligand (Catalog # 9188-4L) co-stimulates IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 5-30 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9188-4L
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9188-4L/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: 4-1BB Ligand/TNFSF9

4-1BB Ligand (4-1BBL), also known as CD137L, is a type II transmembrane protein that belongs to the TNF superfamily of molecules and plays an important role in immune response activation (1). The approximately 50 kDa rat 4-1BB Ligand consists of an 82 aa cytoplasmic domain, a 21 aa transmembrane segment, and a 206 aa extracellular domain (ECD) (2). Within the ECD, rat 4-1BB Ligand shares 34% and 86% aa sequence identity with human and mouse 4-1BB Ligand, respectively. 4-1BB Ligand is expressed by activated B cells, monocytes, macrophages, dendritic cells (DC), T cells, lymphoma and multiple myeloma cells, hematopoietic stem cells, early myeloid progenitors, neurons, and astrocytes (3-9). A 26 kDa soluble form of 4-1BB Ligand can be released from the surface of activated cells and retains bioactivity (10). 4-1BB Ligand binds to 4-1BB/TNFRSF9/CD137 on activated CD4+ and CD8+ T cells, thymocytes, and NK cells as well as on monocytes, neutrophils, DC, and eosinophils. In response to 4-1BB Ligand binding, 4-1BB transduces a co-stimulatory signal that promotes the proliferation, activation, and survival of CD4+ and CD8+ T cells (4, 11, 12). T cell co-stimulation through CD28 is important for the initial T cell expansion, while 4-1BB acts later in the response (12, 13). 4-1BB Ligand function supports the survival and responsiveness of memory T cells during viral infection (13-15). Reverse signaling through
4-1BB Ligand on monocytes induces the production of inflammatory cytokines (5). On macrophages, 4-1BB Ligand associates in cis with TLR4 and enhances inflammatory cytokine production in response to TLR4 ligation (6). Its expression on early myeloid progenitor cells limits the development of dendritic cells, monocytes, and B cells (9).

References

  1. Wang, C. et al. (2009) Immunol. Rev. 229:192.
  2. Dong, Q.M. et al. (2005) Acta Biochim. Biophys. Sin. 37:694.
  3. Reali, C. et al. (2003) J. Neurosci. Res. 74:67.
  4. DeBenedette, M.A. et al. (1997) J. Immunol. 158:551.
  5. Langstein, J. et al. (1998) J. Immunol. 160:2488.
  6. Kang, Y.J. et al. (2007) Nat. Immunol. 8:601.
  7. Baessler, T. et al. (2010) Blood 115:3058.
  8. Gullo, C. et al. (2010) PLoS One 5:e10845.
  9. Lee, S.-W. et al. (2008) Nat. Immunol. 9:917.
  10. Salih, H.R. et al. (2001) J. Immunol. 167:4059.
  11. Wen, T. et al. (2002) J. Immunol. 168:4897.
  12. Cannons, J.L. et al. (2001) J. Immunol. 167:1313.
  13. Bertram, E.M. et al. (2002) J. Immunol. 168:3777.
  14. Bukczynski, J. et al. (2004) Proc. Natl. Acad. Sci. USA 101:1291.
  15. Lin, G.H.Y. et al. (2009) J. Immunol. 182:934.

Alternate Names

41BB Ligand, CD137L, TNFSF9

Entrez Gene IDs

8744 (Human); 21950 (Mouse); 353218 (Rat); 102129676 (Cynomolgus Monkey)

Gene Symbol

TNFSF9

UniProt

Q80WE6

Additional 4-1BB Ligand/TNFSF9 Products

Product Documents for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 9188-4L (with carrier)
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Product Specific Notices for Recombinant Rat 4-1BB Ligand/TNFSF9 Protein

For research use only

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