Recombinant Rat B7-H3 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10610-B3

R&D Systems, part of Bio-Techne
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Key Product Details

Source

NS0

Accession #

Q7TPB4.1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all B7-H3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat B7-H3 protein
Rat B7-H3
(Val29-Phe244)
Accession # Q7TPB4.1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val29

Predicted Molecular Mass

50 kDa

SDS-PAGE

66-76 kDa, under reducing conditions

Activity

Measured by its ability to inhibit Mouse CD3 epsilon Antibody induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.54 µg/mL.

Scientific Data Images for Recombinant Rat B7-H3 Fc Chimera Protein, CF

Recombinant Rat B7-H3 Fc Chimera Protein Bioactivity.

Recombinant Rat B7-H3 Fc Chimera (Catalog # 10610-B3) inhibits Mouse CD3 epsilon Antibody (Clone # 145-2C11, MAB484) induced proliferation of Mouse T cells. The ED50 for this effect is 0.06-0.54 µg/mL.

Recombinant Rat B7-H3 Fc Chimera Protein SDS-PAGE.

2 μg/lane of Recombinant Rat B7-H3 Fc Chimera Protein (Catalog # 10610-B3) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 66-76 kDa and 132-152 kDa, respectively.

Formulation, Preparation and Storage

10610-B3
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H3

B7 homolog 3 (B7-H3), also known as CD276 antigen (CD276), is one member among at least 10 members of the B7 family of immune regulatory proteins within the immunoglobulin superfamily (1-3). The B7 family members all display a conserved extracellular fold but share only about 20-40 % amino acid (aa) sequence identity. Rat B7-H3 consists of an extracellular domain (ECD) containing one V-like and one C-like Ig domain, a helical single-pass type I transmembrane domain, and a cytoplasmic domain. Two isoforms in the ECD of B7-H3 resulting from gene duplication and differential splicing have been identified: one containing four-Ig-like domains (main isoform in humans) and one containing two-Ig-like domains (only isoform in mice) (4, 5). Soluble forms of B7-H3 can result from proteinase cleavage of the isoform with two-Ig-like domains (3). Within the ECD, mature rat B7-H3 shares 92% and 98% aa sequence identity with human and mouse B7-H3, respectively. Human B7-H3 is not expressed on resting B cells, T cells, monocytes or dendritic cells, but is induced on dendritic cells and monocytes by inflammatory cytokines (6, 8). B7-H3 is also overexpressed in numerous cancers including bladder, breast and melanoma (9). Unlike other B7 family members, human B7-H3 does not bind any known members of the CD28 family of immunoreceptors and its receptor has yet to be identified. However, B7-H3 has been shown to bind an unidentified counter-receptor on activated T cells to costimulate the proliferation of CD4+ or CD8+ T cells (10). B7-H3 has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production (6-8, 10).

References

  1. Dong, P. et al. (2018) Front. Oncol. 8:264.
  2. Castellanos, J. et al. (2017) Am. J. Clin. Exp. Immunol. 6:66.
  3. Ni, L. and Dong, C. (2017) Mol. Cancer. Ther. 16:1203.
  4. Shi, T. et al. (2019) Cell Death and Disease. 10: 308.
  5. Tang, X. et al. (2019) Mol. Ther. Oncolytics. 14:279.
  6. Chapoval, A.I. et al. (2001) Nat. Immunol. 2:269.
  7. Coyle, A. and J. Gutierrez-Ramos (2001) Nat. Immunol. 2:203.
  8. Prasad, D.V. et al. (2004) J Immunol. 173:2500.
  9. Dong, P. et al. (2018) Front Oncol. 8:264.
  10. Suh, W.K. et al. (2003) Nat Immunol. 4:899.

Long Name

B7 Homolog 3

Alternate Names

B7H3, CD276

Entrez Gene IDs

80381 (Human); 102657 (Mouse); 315716 (Rat); 102131295 (Cynomolgus Monkey)

Gene Symbol

CD276

UniProt

Q7TPB4.1

Additional B7-H3 Products

Product Documents for Recombinant Rat B7-H3 Fc Chimera Protein, CF

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