Recombinant Rat CD19 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10525-CD

R&D Systems, part of Bio-Techne
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10525-CD-050
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Key Product Details

Source

CHO

Accession #

NP_001013255.2

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD19 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat CD19 protein
Rat CD19
(Arg19-Gly287)
Accession # NP_001013255.2		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg19

Predicted Molecular Mass

57 kDa

SDS-PAGE

80-95 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat CD19 Fc Chimera(Catalog # 10525-CD) is immobilized at 5 µg/mL,100 µL/well, recombinant biotinylated human CD81 Fc Chimera binds with an ED50 of 10-60 μg/mL.

Formulation, Preparation and Storage

10525-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD19

CD19, also known as B4, is a transmembrane glycoprotein of the immunoglobulin superfamily that plays a central role in B cell activation and humoral immune responses (1, 2). CD19 consists of an extracellular domain (ECD) with two C2-type Ig-like domains, a transmembrane segment, and a cytoplasmic domain with nine tyrosine residues, 3 of which are critical for function (1,2). Within the mature ECD, rat CD19 shares 57% and 88% amino acid sequence identity with human and mouse CD19, respectively. CD19 is expressed throughout B cell development from pre‑B cells through mature B cells, and it is commonly used as a B cell lineage marker (1, 2). It is required for the responsiveness of mature B cell to antigen stimulation, germinal center development, and antibody affinity maturation (1, 2). CD19 associates with the B cell antigen receptor (BCR), CD81, CD38, CD21, CD22, and IFITM1/CD225/Leu-13 (1, 3). These associations enable CD19 to amplify B cell signaling and reduce the threshold for antigen stimulation through the BCR (1, 3). CD19 polymorphisms and up-regulation can lead to the development of autoimmunity by promoting autoantibody production (2). CD19+ target cells can be recognized and killed by T cells that express anti-CD19 chimeric antigen receptors (CARs) (4). This interaction has been shown to be beneficial in the treatment of various B-cell malignancies, such as leukemias and lymphomas (4, 5).

References

  1. Wang, K. et al. (2012) Exp. Hematol Oncol. 1:36.
  2. Del Nargo, C.J. et al. (2005) Immunol Res. 31:229.
  3. Yu, F. et al. (2010) J Neurooncol. 103:187.
  4. Kochenderfer, J. et al. (2015) J Clin Oncol. 33:540.
  5. Lee, D. et al. (2015) Lancet. 385:517.

Alternate Names

CD19, CVID3, Leu-12

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Gene Symbol

CD19

UniProt

NP_001013255.2

Additional CD19 Products

Product Documents for Recombinant Rat CD19 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Rat CD19 Fc Chimera Protein, CF

For research use only

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