Recombinant Rat CD34 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9654-CD

R&D Systems, part of Bio-Techne
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9654-CD-050
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Key Product Details

Source

NS0

Accession #

NP_001100672

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

View all CD34 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat CD34 protein
Rat CD34
(Glu37-Leu292)
Accession # NP_001100672		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu37

Predicted Molecular Mass

55 kDa

SDS-PAGE

98-111 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9654-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD34

CD34 is a glycosylated type I transmembrane protein; it was discovered as a hematopoietic cell-surface antigen (1, 2, 3). Rat CD33 cDNA encodes a 386 amino acid (aa) precursor that contains a 36 aa signal sequence, a 256 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 74 aa cytoplasmic domain. Within the ECD, rat CD34 shares 81% and 52% aa sequence identity with mouse and human CD34, respectively. This single-pass sialomucin‑like transmembrane protein is heavily glycosylated and phosphorylated by Protein kinase C (PKC) (4, 5). CD34 is found on multipotent precursors, bone marrow stromal cells, embryonic fibroblasts, vascular endothelia, as well as some populations of mesenchymal stem cells, and tumor cell lines, and it is a common marker for diverse progenitors (6). CD34 is involved in the adhesion of stem cells to the bone marrow extracellular matrix or to stromal cells.

References

  1. Civin C.I. et al. (1984) J. Immunol. 133:157.
  2. Katz F. et al. (1985) Leuk. Res. 9:191.
  3. Andrews R.G. et al. (1986) Blood 67:842.
  4. Young P.E. et al. (1995) Blood 85:96.
  5. Krause D.S. et al. (1996) Blood 87:1.
  6. Sidney L.E. et al. (2014) Stem Cells 32:1380.

Alternate Names

CD34, HPCA1

Entrez Gene IDs

947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)

Gene Symbol

CD34

UniProt

NP_001100672

Additional CD34 Products

Product Documents for Recombinant Rat CD34 Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Rat CD34 Fc Chimera Protein, CF

For research use only

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