Recombinant Rat CNTF Protein
R&D Systems, part of Bio-Techne | Catalog # 557-NT
Key Product Details
Product Specifications
Source
E. coli-derived rat CNTF protein
Ala2-Met200
Ala2-Met200
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala2
Predicted Molecular Mass
22.8 kDa
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 3-15 ng/mL.
Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95.
The ED50 for this effect is 0.1-0.3 ng/mL.
The ED50 for this effect is 3-15 ng/mL.
Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95.
The ED50 for this effect is 0.1-0.3 ng/mL.
Reviewed Applications
Read 1 review rated 5 using 557-NT in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 557-NT
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 557-NT/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CNTF
The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL‑6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. For a list of CNTF references, see the R&D Systems’ cytokine catalog.
Long Name
Ciliary Neurotrophic Factor
Alternate Names
ciliary neurotrophic factor, HCNTF
Gene Symbol
CNTF
UniProt
Additional CNTF Products
Product Documents for Recombinant Rat CNTF Protein
Product Specific Notices for Recombinant Rat CNTF Protein
For research use only
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