Recombinant Rat DLL1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3970-DL

R&D Systems, part of Bio-Techne
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3970-DL-050
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Key Product Details

Source

CHO

Accession #

P97677

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all DLL1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat DLL1 protein
Rat DLL-1
(Ser22 - Ser534)
Accession # P97677		 IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser22

Predicted Molecular Mass

82.6 kDa (monomer)

SDS-PAGE

90-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When rrNotch-1 (Catalog # 1057-TK) is coated at 5 μg/mL, rrDLL-1 binds with an apparent KD <5 nM.

Reviewed Applications

Read 1 review rated 5 using 3970-DL in the following applications:

Formulation, Preparation and Storage

3970-DL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL1

Delta-like protein 1 (DLL1) is a 90 ‑ 100 kDa type I transmembrane protein in the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature rat DLL1 consists of a 520 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF‑like repeats, a 23 aa transmembrane segment, and a 154 aa cytoplasmic domain (1). Within the ECD, rat DLL1 shares 90% and 95% aa sequence identity with human and mouse DLL1, respectively. It shares 26%, 36%, and 53% aa sequence identity with rat DLL2, 3, and 4, respectively. The ADAM9, 12, or 17‑ mediated proteolysis of DLL1 releases a 60 kDa ECD fragment and regulates the Notch-dependent proliferation of hematopoietic and myogenic progenitor cells (2 ‑ 4). The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus (5). DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1 (6). DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, lymphocyte differentiation, and the maintenance of neural and myogenic progenitor cells (4, 7 ‑ 13). The up‑regulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis (14). DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression (15 ‑ 16).

References

  1. Bettenhausen, B. et al. (1995) Development 121:2407.
  2. Dyczynska, E. et al. (2007) J. Biol. Chem. 282:436.
  3. Karanu, F.N. et al. (2001) Blood 97:1960.
  4. Sun, D. et al. (2008) J. Cell Sci. 121:3815.
  5. Ikeuchi, T. and S.S. Sisodia (2003) J. Biol. Chem. 278:7751.
  6. Mizuhara, E. et al. (2005) J. Biol. Chem. 280:26499.
  7. Takahashi, Y. et al. (2003) Development 130:4259.
  8. Teppner, I. et al. (2007) BMC Dev. Biol. 7:68.
  9. Kiernan, A.E. et al. (2005) Development 132:4353.
  10. Schmitt, T.M. and J.C. Zuniga-Pflucker (2002) Immunity 17:749.
  11. Hozumi, K. et al. (2004) Nat. Immunol. 5:638.
  12. Shimojo, H. et al. (2008) Neuron 58:52.
  13. Schuster-Gossler, K. et al. (2007) Proc. Natl. Acad. Sci. 104:537.
  14. Limbourg, A. et al. (2007) Circ. Res. 100:363.
  15. Purow, B.W. et al. (2005) Cancer Res. 65:2353.
  16. Gray, G.E. et al. (1999) Am. J. Pathol. 154:785.

Long Name

Delta-like 1

Alternate Names

Delta 1

Entrez Gene IDs

28514 (Human); 13388 (Mouse); 84010 (Rat)

Gene Symbol

DLL1

UniProt

P97677

Additional DLL1 Products

Product Documents for Recombinant Rat DLL1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Rat DLL1 Fc Chimera Protein, CF

For research use only

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