Recombinant Rat FABP1/L-FABP Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9468-CL

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9468-CL-100
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

E. coli

Accession #

P02692

Conjugate

Unconjugated

View all FABP1/L-FABP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived rat FABP1/L-FABP protein
Met HHHHHH GS Rat FABP1/L-FABP
(Met1-Ile127)
Accession # P02692
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Bioassay data are not available.

Formulation, Preparation and Storage

9468-CL
Formulation Supplied as a 0.2 μm filtered solution in PBS, DTT and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 6 months from date of receipt, 2 to 8 °C as supplied.
  • 3 months, 2 to 8 °C under sterile conditions after opening.

Background: FABP1/L-FABP

Fatty acid binding proteins (FABP) are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner and are involved in intracellular lipid transport. All FABPs bind free fatty acids, cholesterol, and retinoids, which differ in their selectivity, affinity and binding mechanism (1). Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis (2). FABPs are structurally conserved, consisting of a water-filled, ligand-binding pocket surrounded by ten anti-parallel beta-barrel structures, capped by an N-terminal helix-turn-helix motif. The helical N-terminus is involved in the regulation of FA transfer from membranes (3). FABP1, also known as liver FABP (L-FABP) is highly expressed in the liver, intestine, kidney and lung (1). FABP1 binds free fatty acids and their co-enzyme A derivatives. FABP1 is unique among other members in FABP family, attributed to its ability to bind multiple ligands at once. It has a larger solvent-accessible core in comparison to other FABPs, and this allows for more diverse binding to substrates (1). Rat FABP1 is 127 amino acids (aa) in length. It is a two beta-sheet molecule that contains an acetylated initiating methionine. Full-length mouse FABP1 shares 94% and 82% aa identity with rat and human FABP1, respectively (4).

References

  1. Smathers, R. et al. (2011) Hum. Genomics. 5(3):170.
  2. Furuhashi, M. et al. (2008) Nat. Rev. Drug Discov. 7(6):489.
  3. Storch, J. et al. (2010) J. Biol. Chem. 285(43):32679.
  4. Sweetser, D., et al. (1987) J. Biol. Chem. 262(33):16060.

Long Name

Fatty Acid-Binding Protein 1

Alternate Names

L-FABP, LFABP

Entrez Gene IDs

2168 (Human); 14080 (Mouse); 24360 (Rat)

Gene Symbol

FABP1

UniProt

P02692

Additional FABP1/L-FABP Products

Product Documents for Recombinant Rat FABP1/L-FABP Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat FABP1/L-FABP Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.