Recombinant Rat Fc gamma RIIB/CD32b Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9666-CD

R&D Systems, part of Bio-Techne
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9666-CD-100
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Key Product Details

Source

NS0

Accession #

Q63203

Conjugate

Unconjugated

Applications

Bioactivity

View all Fc gamma RIIB/CD32b Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Fc gamma RIIB/CD32b protein
His32-Pro212, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His32 & Gly34

Predicted Molecular Mass

21 kDa

SDS-PAGE

33-45 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat Fc gamma RIIB/CD32b is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG. The concentration of Biotinylated Rat IgG that produces 50% of the optimal binding response is 0.5-3 μg/mL.

Scientific Data Images for Recombinant Rat Fc gamma RIIB/CD32b Protein, CF

Recombinant Rat Fc gamma RIIB/CD32b Protein Bioactivity

Recombinant Rat Fc gamma RIIB/CD32b Protein Bioactivity

When Recombinant Rat Fc gamma RIIB/CD32b (Catalog # 9666-CD) is immobilized on an anti-His Tag Antibody coated plate, it binds Biotinylated Rat IgG with an ED50 of 0.5-3 μg/mL.

Formulation, Preparation and Storage

9666-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIB/CD32b

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Fc gamma RII A and C contain activating cytoplasmic ITAM sequences, while Fc gamma RIIB contains an inhibitory cytoplasmic ITIM sequence. The Fc gamma RI proteins are high affinity receptors (~10-8-10-9 M) capable of binding monomeric IgG, whereas the Fc gamma RII and RIII proteins bind IgG with lower affinities (~10-6-10-7 M) and only recognize IgG aggregates surrounding multivalent antigens (1). The mature rat Fc gamma RIIB consists of a 181 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 52 aa cytoplasmic domain. Within the ECD, rat Fc gamma RIIB shares 58% and 78 % aa sequence identity with human and mouse Fc gamma RIIB, respectively. It binds to both cynomolgus and human IgG subclasses 1-4 (2). Fc gamma RIIB is expressed on B cells, monocytes, dendritic cells, neutrophils, mast cells, and basophils (1). Ligation of Fc gamma RIIB triggers signaling that suppresses B cell expansion, plasma cell differentiation, production of autoimmune rheumatoid factors, and down-regulation of TLR4 with reduced LPS responsiveness (3-6). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (7).

References

  1. Nimmerjahn, F. and J.V. Ravetch (2008) Nat. Rev. Immunol. 8:34.
  2. Warncke, M. et al. (2012) J. Immunol. 188:4405.
  3. Takai, T. et al. (1996) Nature 379:346.
  4. Karnell, J.L. et al. (2014) J. Immunol. 192:1480.
  5. Moll, T. et al. (2004) J. Immunol. 173:4724.
  6. Zhang, Y. et al. (2009) J. Immunol. 182:554.
  7. Ravetch, J. and L. Lanier (2000) Science 290:84.

Long Name

Fc gamma Receptor II

Alternate Names

CD32b, FCGR2B, FcgRIIB, FCRIIB

Entrez Gene IDs

2213 (Human); 14130 (Mouse); 289211 (Rat); 102139530 (Cynomolgus Monkey)

Gene Symbol

FCGR2B

UniProt

Q63203

Additional Fc gamma RIIB/CD32b Products

Product Documents for Recombinant Rat Fc gamma RIIB/CD32b Protein, CF

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