Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9596-CD

R&D Systems, part of Bio-Techne
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9596-CD-100
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Key Product Details

Source

NS0

Accession #

NP_997486

Conjugate

Unconjugated

Applications

Bioactivity

View all Fc gamma RIIIA/CD16a Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Fc gamma RIIIA/CD16a protein
Gly21-Pro200, with a C-ternimal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala20 and Gly21

Predicted Molecular Mass

21 kDa

SDS-PAGE

24-36 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Rat IgG is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Rat Fc gamma RIIIA/CD16a that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.

Scientific Data Images for Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF

Recombinant Rat Fc gamma RIIIA/CD16a Protein Bioactivity

Recombinant Rat Fc gamma RIIIA/CD16a Protein Bioactivity

When Rat IgG is immobilized at 2 μg/mL, 100 μL/well, Recombinant Rat Fc gamma RIIIA/CD16a (Catalog # 9596-CD) binds with an ED50 of 0.1-0.6 μg/mL.

Formulation, Preparation and Storage

9596-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RIIIA/CD16a

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma Rs, based on close relationships in their extracellular domains: Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). Fc gamma RIII has two isoforms, Fc gamma RIIIA and Fc gamma RIIIB. Fc gamma RIII, the only Fc gamma R found on natural killer cells, is also expressed on macrophages, neutrophils, and mast cells (1-3). Unlike Fc gamma RI which binds IgG with high affinity, Fc gamma RIIIA is a low affinity receptor that recognizes IgG as aggregates surrounding multivalent antigens during late immune responses (4, 5). The mature protein consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fc gamma RIIIA shares 83% and 63% amino acid sequence identity with mouse and human Fc gamma RIIIA, respectively.

References

  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today. 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Chenoweth, A.M. et al. (2015) Immunol. Rev. 268:175.

Long Name

Fc gamma Receptor III A

Alternate Names

CD16a, FCGR3A, FcgRIIIA

Entrez Gene IDs

2214 (Human); 304966 (Rat)

Gene Symbol

FCGR3A

UniProt

NP_997486

Additional Fc gamma RIIIA/CD16a Products

Product Documents for Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF

For research use only

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