Recombinant Rat IL-17A/F Heterodimer Protein

R&D Systems, part of Bio-Techne | Catalog # 9340-IL

R&D Systems, part of Bio-Techne
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Carrier Free
9340-IL-025/CF
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With Carrier
9340-IL-025
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Key Product Details

Source

CHO

Accession #

Q61453 (IL-17A) & Q5BJ95 (IL-17F)

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-17A/F Heterodimer Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived rat IL-17A/F Heterodimer protein
Rat IL-17A
(Ala18-Ser150 (Ile46Leu))
Accession # Q61453 
Rat IL-17F
(Arg29-Ala161)
Accession # Q5BJ95
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala18 (IL-17A) & Arg29 (IL-17F)

Predicted Molecular Mass

15 kDa (IL-17A) & 15 kDa (IL-17F)

SDS-PAGE

12-30 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811.
The ED50 for this effect is 3-18 ng/mL.

Scientific Data Images for Recombinant Rat IL-17A/F Heterodimer Protein

Recombinant Rat IL-17A/F Heterodimer Protein Bioactivity

Recombinant Rat IL-17A/F Heterodimer Protein Bioactivity

Recombinant Rat IL‑17A/F Heterodimer (Catalog # 9340-IL) induces IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 3-18 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9340-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 9340-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17A/F Heterodimer

Rat IL-17A/F is an approximately 37 kDa, secreted, disulfide-linked glycosylated heterodimeric protein comprised of two members of the IL-17 family of cytokines, IL-17A and IL-17F (1, 2). Members of this family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors such as TGF-beta (1). Although most cysteine knot superfamily members use three intrachain disulfide bonds to create a knot, IL-17 family molecules generate the same structural form with only two disulfide links (1, 3-5). Mature rat IL-17A and IL-17F share 60% and 59% amino acid sequence identity with human and 61% and 90% with mouse IL-17A and IL-17F, respectively. They also share 50% aa sequence identity with each other (1). IL-17A/F heterodimer and the IL-17A and IL-17F homodimers are produced by IL-23 activated Th17 cells (1, 6-10, 14). The widely expressed receptors IL-17 RA and IL-17 RC form a heterodimer for the binding of
IL-17A and IL-17F, as well as the heterodimeric IL-17A/F (6, 11, 12). IL-17A/F is a biologically active protein that induces chemokine production and airway neutrophilia with intermediate potency between IL-17A (most potent) and IL-17F (least potent) (7, 12). It is up-regulated in immune cells during inflammatory arthritis and contributes to disease severity (13).

References

  1. Wright, J.F. et al. (2007) J. Biol. Chem. 282:13447.
  2. Chang, S.H. and C. Dong (2007) Cell Res. 17:435.
  3. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265.
  4. Kolls, J.K. and A. Linden (2004) Immunity 21:467.
  5. Gu, C. et al. (2013) Cytokine 64:477.
  6. Cheung, P.F.Y. et al. (2008) J. Immunol. 180:5625.
  7. Liang, S.C. et al. (2007) J. Immunol. 179:7791.
  8. Ouyang, W. et al. (2008) Immunity 28:454.
  9. Steinman, L. (2007) Nat. Med. 13:139.
  10. Hunter, C.A. (2005) Nat. Rev. Immunol. 5:521.
  11. Kuestner, R.E. et al. (2007) J. Immunol. 179:5462.
  12. Wright, J.F. et al. (2008) J. Immunol. 181:2799.
  13. Sarkar, S. et al. (2014) Clin. Exp. Immunol. 177:652.
  14. Spolski, R. et al. (2009) Eur. J. Immunol. 3: 658.

Long Name

Interleukin 17 A/F

Alternate Names

CTLA8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, Cytotoxic T-Lymphocyte-Associated Protein 8, IL17, IL-17, IL-17A, interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8), interleukin 17A, interleukin-17A

UniProt

Q61453 (IL-17A) & Q5BJ95 (IL-17F)

Additional IL-17A/F Heterodimer Products

Product Documents for Recombinant Rat IL-17A/F Heterodimer Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Rat IL-17A/F Heterodimer Protein

For research use only

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