Recombinant Rat IL-7 Protein

R&D Systems, part of Bio-Techne | Catalog # 7857-RL

R&D Systems, part of Bio-Techne
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Carrier Free
7857-RL-010/CF
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With Carrier
7857-RL-010
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Key Product Details

Source

E. coli

Accession #

NP_037242

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived rat IL-7 protein
Asp26-Ile154, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

15 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
The ED50 for this effect is 0.1-0.6 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7857-RL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 7857-RL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-7

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Rat IL‑7 cDNA encodes 154 amino acids (aa) that include a 25 aa signal peptide. Mature rat IL‑7 shares approximately 88% aa sequence identity with mouse IL‑7 and 58‑60% with human, equine, bovine, ovine, porcine, feline and canine IL‑7. Human and mouse IL‑7 exhibit cross‑species activity (2, 3). IL‑7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL‑7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL‑7 signals through a complex of the IL‑7 Receptor alpha subunit (IL‑7 R alpha, also known as CD127) with the common gamma chain ( gammac) (1). The gammac is also a subunit of the receptors for IL‑2, ‑4, ‑9, ‑15, and ‑21 (1). IL‑7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive naïve and memory T cells, but undergoes IL‑7‑mediated down‑regulation and shedding during antigen‑driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6‑11). IL‑7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl‑2 (9‑11). It is required for optimal T cell‑dendritic cell interaction (6). IL‑7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL‑7 activation of IL‑7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL‑7 functions in both mouse and human pro‑B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

References

  1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
  2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
  4. Namen, A.E. et al. (1988) Nature 333:571.
  5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
  6. Saini, M. et al. (2009) Blood 113:5793.
  7. Park, J.H. et al. (2004) Immunity 21:289.
  8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
  11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
  12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
  15. Nodland, S.E. et al. (2011) Blood 118:2116.

Long Name

Interleukin 7

Alternate Names

IL7, Lymphopoietin-1, PBGF

Entrez Gene IDs

3574 (Human); 16196 (Mouse); 25647 (Rat)

Gene Symbol

IL7

UniProt

NP_037242 (Rat)

Additional IL-7 Products

Product Documents for Recombinant Rat IL-7 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 7857-RL (with carrier)
Download SDS 7857-RL/CF (carrier free)

Product Specific Notices for Recombinant Rat IL-7 Protein

For research use only

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