Recombinant Rat LIX Protein
R&D Systems, part of Bio-Techne | Catalog # 543-RL
Key Product Details
Product Specifications
Source
E. coli-derived rat LIX protein
Ala38-Arg125 & Ala38-Lys117
Ala38-Arg125 & Ala38-Lys117
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala38
Predicted Molecular Mass
10.1 kDa
Activity
Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with human CXCR2.
The ED50 for this effect is 0.03-0.15 µg/mL.
The ED50 for this effect is 0.03-0.15 µg/mL.
Reviewed Applications
Read 1 review rated 4 using 543-RL in the following applications:
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 543-RL
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 25 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 543-RL/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: LIX
LPS-induced CXC chemokine (LIX) is a chemokine originally cloned from LPS-stimulated mouse fibroblasts. Rat LIX shares approximately 74% amino acid (aa) sequence identity with mouse LIX and is likely the rat orthologue to mouse LIX. Rat LIX cDNA encodes a 130 aa residue precursor with a predicted 37 aa residue signal peptide and a 93 aa residue mature protein. Among human CXC chemokines, rat LIX is most closely related to human GCP-2 and ENA-78. Rat LIX also differs from these two human proteins by having an extended carboxy-terminus. The amino-terminal 115 residues of rat LIX shares 59% and 53% aa sequence homology with human GCP-2 and ENA-78, respectively. It is not clear if LIX should be considered an orthologue of GCP-2 or ENA-78. Yet, mouse LIX was alternatively named mouse GCP-2.
Purified recombinant rat LIX is C-terminally truncated. This is consistent with the observation that natural mouse LIX purified from fibroblasts and epithelial cells also contains multiple amino-terminal and carboxy-terminal truncated isoforms. The shorter isoforms of the natural protein were reported to be more active than the longer forms. The bioactivity of rat LIX can be mediated through CXCR2.
References
- Proost, P. et al. (1993) J. Immunol. 150:1000.
- Smith, J.B. and H.R. Herschman (1995) J. Biol. Chem. 270:16756.
- Rovai, L.E. et al. (1997) J. Immunol. 158:5257.
- Wuyts, A. et al. (1997) J. Immunol. 157:1736.
Alternate Names
LIX
Gene Symbol
CXCL5
UniProt
Additional LIX Products
Product Documents for Recombinant Rat LIX Protein
Product Specific Notices for Recombinant Rat LIX Protein
For research use only
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