Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

R&D Systems, part of Bio-Techne | Catalog # 8897-LR

R&D Systems, part of Bio-Techne
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8897-LR-050
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Key Product Details

Source

NS0

Accession #

NP_001008316

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Lymphotoxin beta R/TNFRSF3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Lymphotoxin beta R/TNFRSF3 protein
Rat Lymphotoxin betaR
(Gln29-Met222)
Accession # NP_001008316		 IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln29 predicted

Predicted Molecular Mass

49 kDa

SDS-PAGE

60-68 kDa, reducing conditions

Activity

Measured by its ability to inhibit Lymphotoxin alpha1/ beta2-induced IL-8 secretion in A375 human melanoma cells. Degli-Esposti, M. et al. (1997) J. Immunol. 158:1756.
The ED50 for this effect is 0.15-0.9 μg/mL.

Formulation, Preparation and Storage

8897-LR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin beta R/TNFRSF3

Lymphotoxin  betaR (LT betaR; also called TNFRSF3, TNF RIII,or TNF Rrp) is a type I transmembrane glycoprotein within the TNF receptor superfamily (1-3). The rat LT betaR cDNA encodes a 415 amino acid (aa) protein that includes a 30 aa signal peptide, a 191 aa extracellular domain (ECD), a 23 aa transmembrane domain, and a 171 aa cytoplasmic domain. The ECD contains four cysteine-rich motifs characteristic of the TNF receptor superfamily (2). Within the ECD, rat LT betaR shares 90% and 66% aa sequence identity with the ECD of mouse and human LT betaR, respectively. Soluble LT betaR, which can be formed by proteolytic cleavage of the ECD, functions as a decoy receptor for LT betaR ligands and is involved in the suppression of autoimmunity (4, 5). LT betaR is constitutively expressed in cells of myeloid lineage and is upregulated during tissue regeneration (6). It is expressed on mesenchymal stromal organizing cells that give rise to primary, secondary, and tertiary lymphoid structures (7-9). Mice deficient in LT betaR fail to form secondary lymphoid structures (4). LT betaR ligands include homotrimers of LIGHT/TNFSF14 and the heterotrimeric Lymphotoxin alpha1/ beta2 (2). Ligand engagement of LT betaR has been shown to induce NF kappaB activation through canonical (IKK) or alternative (NIK/RelB) signaling pathways (5, 10). LT betaR signaling induces production of cytokines (TRANCE/RANK Ligand/TNFSF11, IL-7), chemokines (CXCL8/IL-8, CXCL13/BCL/BCA-1, CCL19/MIP-3 beta, CCL21/6Ckine, CCL2/MCP-1), and adhesion molecules (VCAM-1/CD106, ICAM-1/CD54, MAdCAM) (8, 11). LT betaR is involved in lipid metabolism, atherosclerosis, and intestinal epithelial homeostasis (4, 12, 13). It also regulates cell growth and can initiate inflammation-related carcinogenesis (5, 14).

References

  1. Force, W.R. et al. (1995) J. Immunol. 155:5280.
  2. Remouchamps, C. et al. (2011) Cytokine Growth Factor Rev. 22:301.
  3. Crowe, P.D. et al. (1994) Science 264:707.
  4. Tumanov, A.V. et al. (2007) Curr. Mol. Med. 7:567.
  5. Wolf, M.J. et al. (2010) Oncogene 29:5006.
  6. Ware, C.F. (2005) Annu. Rev. Immunol. 23:787.
  7. Boulianne, B. et al. (2012) Front. Immunol. 3:243.
  8. Mouri, Y. et al. (2011) J. Immunol. 186:5047.
  9. van de Pavert, S.A. and R.E. Mebius (2010) Nat. Rev. Immunol. 10:664.
  10. Bista, P. et al. (2010) J. Biol. Chem. 285:12971.
  11. Mikami, Y. et al. (2014) PLoS One 9:e114791.
  12. Macho-Fernandez, E. et al. (2015) Mucosal Immunol. 8:403.
  13. Browning, J.L. (2012) Mucosal Immunol. 5:228.
  14. Haybaeck, J. et al. (2009) Cancer Cell 16:295.

Long Name

Lymphotoxin beta Receptor

Alternate Names

LTBR, LymphotoxinbR, TNF RIII, TNF Rrp, TNFRSF3

Entrez Gene IDs

4055 (Human); 17000 (Mouse); 297604 (Rat); 102135920 (Cynomolgus Monkey); 712550 (Rhesus Macaque)

Gene Symbol

LTBR

UniProt

NP_001008316

Additional Lymphotoxin beta R/TNFRSF3 Products

Product Documents for Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

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Product Specific Notices for Recombinant Rat Lymphotoxin beta R/TNFRSF3 Fc Chimera, CF

For research use only

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