Recombinant Rat MMR/CD206 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7858-MR

R&D Systems, part of Bio-Techne
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7858-MR-050
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Key Product Details

Source

NS0

Accession #

NP_001099593

Conjugate

Unconjugated

Applications

Bioactivity

View all MMR/CD206 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat MMR/CD206 protein
Leu26-Ala1395, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Leu26

Predicted Molecular Mass

156.4 kDa

SDS-PAGE

150-180 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.

When Mannan is coated at 5 μg/mL (100 μL/well), the concentration of Recombinant Rat MMR/CD206 that produces 50% optimal binding response is 0.1-0.5 μg/mL.

Formulation, Preparation and Storage

7858-MR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MMR/CD206

The MMR (macrophage mannose receptor) is also called MR due to its presence on cells other than macrophages, and is designated CD206, Mrc1 (mannose receptor C type 1), or CLEC13D (C-type lectin domain family 13, member D) (1‑4). CD206 is a 175 kDa endocytic receptor that is expressed on M2 alternatively activated tissue macrophages including tumor-associated macrophages (TAMs), inflammatory dendritic cells in selected lymphoid organs, and liver, splenic, lymphatic, and dermal microvascular endothelial cells (1, 2, 5‑8). The 1463 amino acid (aa) rat CD206 precursor contains a signal sequence (26 aa), an extracellular domain (ECD) containing an N‑terminal cysteine‑rich domain, a fibronectin type II (FNII) domain, eight C‑type lectin domains (CTLDs), and several N‑glycosylation sites (1369 aa), a transmembrane segment and a short (47 aa) cytoplasmic domain (2‑4). Metalloproteinases can mediate the shedding of the soluble ECD (2). The rat CD206 ECD shares 96% aa sequence identity with mouse CD206, and 84‑85% with human, equine, porcine and canine CD206. The cysteine‑rich domain recognizes some pituitary hormones such as LH (luteinizing hormone/lutropin) and TSH (thyroid stimulating hormone/thyrotropin), chondroitin sulfates, and sulfated N‑acetylgalactosamines including sulfo‑Lewisa and ‑Lewisx (1, 7, 9). The FNII domain mediates Ca2+‑independent binding of collagens (2, 10). The CTLDs participate in Ca2+‑dependent recognition of branched sugars with terminal mannose, fucose or N‑acetylglucosamine that occur on many pathogenic microorganisms (7, 11). CD206 internalizes ligands in clathrin‑coated vesicles, sorts them to phagosomes or early endosomes, and recycles to the cell surface (1, 6, 7). CD206 also promotes clearance of glycoproteins that promote allergy or ongoing inflammation, such as lysosomal hydrolases and myeloperoxidases (1, 2, 5‑7). It is involved in T cell polarization and production of pro‑ and anti‑inflammatory cytokines (1, 2). It facilitates peptide presentation on MHC II, and cross‑presentation on MHC I which is important for tumor immunogenicity (1, 2, 12). This function may be blocked by engagement of CD206 on TAMs by tumor mucins (8).

References

  1. Gazi, U. and L. Martinez-Pomares (2009) Immunobiology 214:554.
  2. Martinez-Pomares, L. (2012) J. Leukoc. Biol. 92:1177.
  3. Harris, N. et al. (1992) Blood 80:2363.
  4. Taylor, M.E. et al. (1990) J. Biol. Chem. 265:12156.
  5. Chieppa, M. et al. (2003) J. Immunol. 171:4552.
  6. Figdor, C. et al. (2002) Nat. Rev. Immunol. 2:77.
  7. Taylor, P.R. et al. (2005) Trends Immunol. 26:104.
  8. Allavena, P. et al. (2010) Clin. Dev. Immunol. 2010:547179.
  9. Leteux, C. et al. (2000) J. Exp. Med. 191:1117.
  10. Martinez-Pomares, L. et al. (2006) Eur. J. Immunol. 36:1074.
  11. Taylor, M.E. et al. (1992) J. Biol. Chem. 267:1719.
  12. Singh S.K. et al. (2011) Eur. J. Immunol. 41:916.

Long Name

Macrophage Mannose Receptor

Alternate Names

CD206, CLEC13D, MRC1

Entrez Gene IDs

4360 (Human); 17533 (Mouse); 291327 (Rat)

Gene Symbol

MRC1

UniProt

NP_001099593 (Rat)

Additional MMR/CD206 Products

Product Documents for Recombinant Rat MMR/CD206 Protein, CF

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Product Specific Notices for Recombinant Rat MMR/CD206 Protein, CF

For research use only

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