Recombinant Rat Neuroligin 1/NLGN1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 4340-NL

R&D Systems, part of Bio-Techne
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4340-NL-050
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Key Product Details

Source

NS0

Accession #

Q62765 (isoform 4)

Conjugate

Unconjugated

Applications

Bioactivity

View all Neuroligin 1/NLGN1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived rat Neuroligin 1/NLGN1 protein
Met1-Leu676, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln46 predicted

Predicted Molecular Mass

71 kDa

SDS-PAGE

85-105 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When recombinant rat Neuroligin 1 is immobilized at 5 μg/mL, biotinylated recombinant human Neurexin 1 beta Fc Chimera binds with an apparent KD <20 nM. 

Formulation, Preparation and Storage

4340-NL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuroligin 1/NLGN1

Neuroligin 1 (NLGN1 or NL1) is one of several type I transmembrane Neuroligin proteins that play an important role in synaptic development (1). Mature rat Neuroligin 1 is an N- and O-glycosylated 120 kDa molecule that consists of a 652 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 125 aa cytoplasmic tail (2). Insertion of 20 aa at splice site A and/or 9 aa at splice site B within the ECD results in NLGN1A, NLGN1B, or NLGN1AB isoforms while the NLGN1(-) isoform has neither insertion (3). This recombinant protein (NLGN1B) contains the insert at splice site B but not the insert at splice site A. Within the ECD, rat NLGN1B shares 99% aa sequence identity with comparable regions of human and mouse Neuroligin 1. Neuroligin 1 expression is restricted to postsynaptic nerve terminal membranes where it associates into dimers and tetramers (5 - 7). NLGN1B and NLGN1AB are enriched at excitatory (glutamatergic) contacts, whereas NLGN1A is enriched at inhibitory (GABAergic) contacts and NLGN1(-) is expressed at both (3). The interaction of Neuroligin 1 with presynaptic membrane Neurexins promotes the maturation of presynaptic as well as postsynaptic structures (3, 4, 8 - 11). Neuroligin 1 isoforms are selective in which Neurexin isoforms they bind: NLGN1B and NLGN1AB preferentially interact with Neurexin 1 beta lacking the splice site 4 insert, while NLGN1A and NLGN1(-) bind Neurexin 1 alpha and Neurexin 1 beta irrespective of the SS4 insert (12). Neurexin 1 beta (-SS4) additionally binds to LRRTM2 to trigger a second mechanism that promotes excitatory synapse development (13, 14). Neuroligin 1 mediated synaptogenesis is also enhanced by its direct binding to Thrombospondin 1 (15).

References

  1. Sudhof, T.C. (2008) Nature 455:903.
  2. Ichtchenko, K. et al. (1995) Cell 81:435.
  3. Chih, B. et al. (2006) Neuron 51:171.
  4. Dean, C. et al. (2003) Nat. Neurosci. 7:708.
  5. Comoletti, D. et al. (2006) Biochemistry 45:12816.
  6. Berninghausen, O. et al. (2007) J. Neurochem. 103:1855.
  7. Song, J.-Y. et al. (1999) Proc. Natl. Acad. Sci. 96:1100.
  8. Wittenmayer, N. et al. (2009) Proc. Natl. Acad. Sci. 106:13564.
  9. Jung, S.-Y. et al. (2010) Proc. Natl. Acad. Sci. 107:4710.
  10. Chubykin, A.A. et al. (2007) Neuron 54:919.
  11. Chih, B. et al. (2005) Science 307:1324.
  12. Boucard, A.A. et al. (2005) Neuron 48:229.
  13. Siddiqui, T.J. et al. (2010) J. Neurosci. 30:7495.
  14. Ko, J. et al. (2009) Neuron 64:791.
  15. Xu, J. et al. (2010) Nat. Neurosci. 13:22.

Alternate Names

Nlg, NLGN1

Entrez Gene IDs

22871 (Human); 192167 (Mouse); 116647 (Rat)

Gene Symbol

NLGN1

UniProt

Q62765 (isoform 4)

Additional Neuroligin 1/NLGN1 Products

Product Documents for Recombinant Rat Neuroligin 1/NLGN1 Protein, CF

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Product Specific Notices for Recombinant Rat Neuroligin 1/NLGN1 Protein, CF

For research use only

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