Recombinant S. marcescens NucA nuclease Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10038-NA

R&D Systems, part of Bio-Techne
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10038-NA-100
10038-NA-020
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Key Product Details

Source

E. coli

Accession #

P13717

Conjugate

Unconjugated

Applications

Enzyme Activity

View all NucA nuclease Proteins and Enzymes »

Product Specifications

Source

E. coli-derived s. marcescens NucA nuclease protein
Met1-Asn266

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Predicted Molecular Mass

28.9 kDa

SDS-PAGE

29 kDa, reducing conditions

Activity

Measured by its ability to hydrolyze DNA from salmon testes.
The specific activity is >130,000 pmol/min/ug, as measured under the described conditions.

Reviewed Applications

Read 2 reviews rated 4.5 using 10038-NA in the following applications:

Scientific Data Images for Recombinant S. marcescens NucA nuclease Protein, CF

NucA nuclease Digestion of DNA.

NucA nuclease Digestion of DNA.

2 μg plasmid DNA was incubated with increasing amounts (ng/mL) of RecombinantS. marcescensNucA nuclease (Catalog # 10038-NA) in 50 mM Tris (pH 8.0) and 1 mM MgCl2 for 30 minutes at room temperature. Samples were analyzed by agarose gel electrophoresis and DNA was visualized by ethidium bromide staining.

Formulation, Preparation and Storage

10038-NA
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: NucA nuclease

Serratia marcescens endonuclease, is a non-specific nuclease that degrades both single- and double-stranded nucleic acids, including DNA and RNA, but exhibits no proteolytic activity (1); therefore, it is ideal for the removal of nucleic acid contaminants from protein samples and applications where complete digestion of nucleic acids is desirable. NucA is also commonly used in bioprocessing applications to reduce viscosity of samples caused by genomic DNA. The optimum pH for enzyme activity is found to be 8.0-9.2. It hydrolyzes internal phosphodiester bonds between nucleotides in nucleic acids to produce 5'-monophosphate oligonucleotides of 3-8 bases in length (2). The active enzyme is a homodimer with two disulfide bonds in each monomer that are crucial to the enzyme activity and stability (3). The absolute activity of the recombinant enzyme is measured by a phosphatase coupled assay (4), where the 5'-phosphate of oligosaccharides generated by the enzyme is further released by non-specific alkaline phosphatase and quantitated by Malachite reagents (5).

References

  1. Benedik, MJ and Strych, U. (1998) FEMS Microbiol Lett. 165:1.
  2. Nestle, M, et al. (1999) J. Biol. Chem. 274:825.
  3. Ball, T.K. et al. (1992) Nucleic Acids Res. 20:4971.
  4. Wu, Z.L. et al. (2011) Glycobiology 21:727.
  5. Van Veldhoven, P.P. and G.P. Mannaerts (1987) Anal. Biochem. 161:45.

Long Name

NucA nuclease

Alternate Names

DcD

Entrez Gene IDs

23387471 (Bacterial)

Gene Symbol

SMDB11_RS05280

UniProt

P13717

Additional NucA nuclease Products

Product Documents for Recombinant S. marcescens NucA nuclease Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant S. marcescens NucA nuclease Protein, CF

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