Recombinant SARS-CoV-2 Nucleocapsid His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10898-CV

R203K G204R
R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
10898-CV-100
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

YP_009724397.2

Conjugate

Unconjugated

Applications

Bioactivity

View all Nucleocapsid Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived sars-cov-2 Nucleocapsid protein
Met1-Ala419 (Arg203Lys, Gly204Arg), with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser2, determined by protein ID

Predicted Molecular Mass

47 kDa

SDS-PAGE

47-53 kDa, under reducing conditions.

Activity

Bioassay data are not available.

Scientific Data Images for Recombinant SARS-CoV-2 Nucleocapsid His-tag Protein, CF

Recombinant SARS-CoV Nucleocapsid His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant SARS-CoV Nucleocapsid His-tag Protein (Catalog # 10898-CV) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 47-53 kDa.

Formulation, Preparation and Storage

10898-CV
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nucleocapsid

SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1).  While the S, E and M proteins build up the viral envelop, the N protein is involved transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid (RNP) (2, 3). The SARS-CoV-2 N protein is a ~45 kDa protein composed of two independent structural domains connected by a linker region. The N-terminal region contains an RNA binding domain, the linker region interacts with the M protein and the C-terminal region contains a self-association domain (2, 3). The SARS-CoV2 N protein shares 91% and 47% amino acid sequence identity with SARS-CoV-1 and MERS N protein, respectively.  The SARS-CoV-2 N protein displays VSR (viral suppressor of RNA interference) activity in mammalian cells (4). In addition, the N protein is an abundant protein during coronavirus infection and displays high immunogenic activity (5, 6), so it has been used to develop serological diagnostic kit for Covid-19 IgM and IgG antibody tests (7). The co-occurring mutations R203K/G204R within the N protein were carried by some high transmissibility variants, including B.1.1.7 and P.1. It was reported that the R203k/G204R mutations contributes to the increased transmission and virulence of the SARS2-Cov-2 variants (8).

References

  1. Wu, F. et al. (2020) Nature 579:265.
  2. Chang, C. K. et al. (2006) J. Biomed. Sci. 13:59.
  3. Hurst, K.R. et al. (2009) J. Virol. 83:7221.
  4. Mu, J. et al. (2020) Sci. China Life Sci. doi: 10.1007/s11427-020-1692-1.
  5. Che, X. Y. et al. (2004) J. Clin. Microbiol. 42:2629.
  6. Guan, M. et al. (2004) Clin. Diagn. Lab. Immunol. 11:287.
  7. Liu, W. et al. (2020) J. Clin. Microbiol. doi: 10.1128/JCM.00461-20.
  8. Wu, H. et al. (2021) bioRxiv https://www.biorxiv.org/content/10.1101/2021.05.24.445386v1.

Long Name

Nucleocapsid Protein

Alternate Names

N Protein

Entrez Gene IDs

918763 (HCoV-229E); 3200423 (HCoV-HKU1); 2943504 (HCoV-NL63); 39105221 (HCoV-OC43); 14254601 (MERS-CoV); 1489678 (SARS-CoV); 43740575 (SARS-CoV-2)

Gene Symbol

N

UniProt

YP_009724397.2

Additional Nucleocapsid Products

Product Documents for Recombinant SARS-CoV-2 Nucleocapsid His-tag Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant SARS-CoV-2 Nucleocapsid His-tag Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.