Recombinant SARS-CoV-2 S GCN4-IZ Alexa Fluor® 488 Protein
R&D Systems, part of Bio-Techne | Catalog # AFG10561
His-tag
Key Product Details
Learn more about FluorokinesTM Fluorescent-Labeled Proteins
Source
HEK293
Accession #
Structure / Form
Non-covalent trimer / multimer, Labeled with Alexa Fluor® 488 via amines
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm
Excitation Wavelength: 488 nm
Emission Wavelength: 515-545 nm
Conjugate
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived sars-cov-2 Spike protein
| SARS-CoV-2 Spike (Val16-Lys1211) (R682S)(R685S)(K986P)(V987P) Accession # YP_009724390.1 |
GCN4-IZ | HHHHHH |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val16
Predicted Molecular Mass
138 kDa
SDS-PAGE
144-175 kDa, under reducing conditions
Activity
Measured
by flow cytometry for its ability to bind HEK293 human embryonic kidney cells
transfected with human ACE-2 at 0.50-2.00 µg/mL (100 µL/well).
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Scientific Data Images for Recombinant SARS-CoV-2 S GCN4-IZ Alexa Fluor® 488 Protein
Detection of ACE-2 on HEK293 Transfectants with Recombinant SARS-CoV-2 Spike (GCN4-IZ) His-tag Alexa Fluor® 488 by Flow Cytometry.
HEK293 human embryonic kidney cells transfected with human ACE-2 were stained with (A) 1 µg/mL (100 µL/well) Recombinant SARS-CoV-2 Spike (GCN4-IZ) His-tag Alexa Fluor® 488 Protein (Catalog # AFG10561) or (B) unstained.
Recombinant SARS-CoV-2 Spike (GCN4-IZ) His-tag Alexa Fluor® 488 Protein SDS-PAGE.
2 μg/lane of Recombinant SARS-CoV-2 Spike (GCN4-IZ) His-tag Alexa Fluor® 488 Protein (Catalog # AFG10561) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 144-175 kDa.Formulation, Preparation and Storage
AFG10561
| Formulation | Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Spike
SARS-CoV-2, which causes the global pandemic coronavirus disease 2019 (Covid-19), belongs to a family of viruses known as coronaviruses that are commonly comprised of four structural proteins: Spike protein (S), Envelope protein (E), Membrane protein (M), and Nucleocapsid protein (N) (1). SARS-CoV-2 Spike Protein (S Protein) is a glycoprotein that mediates membrane fusion and viral entry. The S protein is homotrimeric, with each ~180-kDa monomer consisting of two subunits, S1 and S2 (2). In SARS-CoV-2, as with most coronaviruses, proteolytic cleavage of the S protein into the S1 and S2 subunits is required for activation. The S1 subunit is focused on attachment of the protein to the host receptor while the S2 subunit is involved with cell fusion (3-5). The S protein of SARS-CoV-2 shares 75% and 29% amino acid (aa) sequence identity with the S protein of SARS-CoV-1 and MERS, respectively. The S Protein of the SARS-CoV-2 virus, like the SARS-CoV-1 counterpart, binds Angiotensin-Converting Enzyme 2 (ACE-2), but with much higher affinity and faster binding kinetics through the receptor binding domain (RBD) located in the C-terminal region of S1 (6). Based on structural biology studies, the RBD can be oriented either in the up/standing or down/lying state with the up/standing state associated with higher pathogenicity (7). Polyclonal antibodies to the RBD of the SARS-CoV-2 protein have been shown to inhibit interaction with the ACE-2 receptor, confirming RBD as an attractive target for vaccinations or antiviral therapy (8). It has been demonstrated that the S Protein can invade host cells through the CD147/EMMPRIN receptor and mediate membrane fusion (9).
References
- Wu, F. et al. (2020) Nature 579:265.
- Tortorici, M.A. and D. Veesler (2019). Adv. Virus Res. 105:93.
- Bosch, B.J. et al. (2003). J. Virol. 77:8801.
- Belouzard, S. et al. (2009) Proc. Natl. Acad. Sci. 106:5871.
- Millet, J.K. and G.R. Whittaker (2015) Virus Res. 202:120.
- Ortega, J.T. et al. (2020) EXCLI J. 19:410.
- Yuan, Y. et al. (2017) Nat. Commun. 8:15092.
- Tai, W. et al. (2020) Cell. Mol. Immunol. 17:613
- Wang, K. et al. (2020) Signal Transduct Target Ther. 5:283.
Long Name
Spike Protein
Alternate Names
S Protein
Entrez Gene IDs
Gene Symbol
S
UniProt
Additional Spike Products
Product Documents for Recombinant SARS-CoV-2 S GCN4-IZ Alexa Fluor® 488 Protein
Product Specific Notices for Recombinant SARS-CoV-2 S GCN4-IZ Alexa Fluor® 488 Protein
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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