Recombinant Viral CCI Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 696-CC
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived viral CCI protein
| Viral CCI (Met17-Val258)& (Pro18-Val258) Accession # P19063 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
6-His tag |
| N-terminus | C-terminus | ||
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met17 & Pro18
Predicted Molecular Mass
57 kDa (monomer)
Activity
Measured by its ability to inhibit JE-induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR2A.
The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 20 ng/mL of recombinant mouse JE.
The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 20 ng/mL of recombinant mouse JE.
Scientific Data Images for Recombinant Viral CCI Fc Chimera Protein, CF
Recombinant Viral CCI Fc Chimera Protein Bioactivity
Recombinant Viral CCI Fc Chimera (Catalog # 696-CC) inhibits Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE) induced chemotaxis of BaF3 mouse pro-B cells transfected with human CCR2A. The ED50 for this effect is 0.1-0.5 µg/mL in the presence of 20 ng/mL Recombinant Mouse CCL2/JE/MCP-1.Formulation, Preparation and Storage
696-CC
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CCI
CC-chemokines with high affinity and are termed viral chemokine inhibitor (vCCI). Viral CCI from various poxviruses share multiple stretches of sequence identity and eight conserved cysteine residues (1). The vaccinia virus (strain Lister) vCCI cDNA encodes a 258 amino acid (aa) protein with a putative 17 aa signal peptide (2). Vaccinia virus (strain Lister) vCCI shows greater than 90% aa sequence identity with vCCI from other orthopoxviruses and approximately 40% aa sequence identity with the leporipoxvirus T-1 proteins. vCCI binds with high affinity to many human, mouse, and rat CC-chemokines (1, 3-5). It binds to the same surfaces of these chemokines that are involved in chemokine receptor interactions (4-7). Some CC-chemokines utilize common binding surfaces on vCCI, while others interact with distinct sites on vCCI (8). vCCI inhibits CC-chemokine induced monocyte chemotaxis in vitro but does not block cellular effects induced by CXCL5/ENA-78, CXCL8/IL-8, or CXCL10/IP-10 (3-5). In vivo, vCCI restricts the infiltration of immune cells into sites of inflammation (3, 9, 10) and also the CC-chemokine induced migration of arterial vascular smooth muscle cells (11).
References
- Graham, K.A. et al. (1997) Virology 229:12.
- Patel, A.H. et al. (1990) J. Gen. Virol. 71:2013.
- Buatois, V. et al. (2010) J. Immunol. 185:2544.
- Smith, C.A. et al. (1997) Virology 236:316.
- Lalani, A.S. et al. (1998) Virology 250:173.
- Beck, C.G. et al. (2001) J. Biol. Chem. 276:43270.
- Seet, B.T. et al. (2001) Proc. Natl. Acad. Sci. 98:9008.
- Burns, J.M. et al. (2002) J. Biol. Chem. 277:2785.
- Dabbagh, K. et al. (2000) J. Immunol. 165:3418.
- Reading, P.C. et al. (2003) J. Immunol. 170:1435.
- Spinetti, G. et al. (2004) Arterioscler. Throm. Vasc. Biol. 24:1397.
Long Name
CC Chemokine Inhibitor
UniProt
Additional CCI Products
Product Documents for Recombinant Viral CCI Fc Chimera Protein, CF
Product Specific Notices for Recombinant Viral CCI Fc Chimera Protein, CF
For research use only
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