Recombinant Viral HCV E2 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9146-HC

Hepatitis C Virus 1b Envelope Glycoprotein E2
R&D Systems, part of Bio-Techne
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9146-HC-050
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Key Product Details

Source

HEK293

Accession #

NP_671491

Conjugate

Unconjugated

Applications

Bioactivity

View all HCV E2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived viral HCV E2 protein
Glu384-Glu661, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu384

Predicted Molecular Mass

31 kDa

SDS-PAGE

63-74 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is coated onto a microplate at 2 μg/mL, Recombinant Viral HCV E2 binds with an ED50 of 0.2-1.2 µg/mL.

Scientific Data Images for Recombinant Viral HCV E2 Protein, CF

Recombinant Viral HCV E2 Protein Bioactivity

Recombinant Viral HCV E2 Protein Bioactivity

When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is coated onto a microplate at 2 µg/ml, Recombinant Viral HCV E2 (Catalog # 9146-HC) binds with an ED50 of 0.2-1.2 µg/mL.

Formulation, Preparation and Storage

9146-HC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HCV E2

Glycoprotein E2 is an approximately 60 kDa viral envelope protein encoded by the hepatitis C virus (HCV) genome (1-3). HCV infection is closely associated with the development of hepatocellular carcinoma and cirrhosis of the liver. E2 is a transmembrane molecule expressed as a noncovalent dimer with the E1 glycoprotein (4). It binds to CD81 and CD36/SR-BI on hepatocytes, and glycosylation of E2 is important for this interaction (4-8). The virus also requires target cell expression of the tight junction proteins Claudin-1 and Occludin for infectivity (7, 9, 10). CD81 and Occludin are responsible for the human host cell specificity of HCV (10). E2 binding to CD81 on NK cells inhibits cytokine production and cytolytic activity (11, 12). In contrast, E2 binding to CD81 on T cells provides a costimulatory signal (11, 12). On plasmacytoid dendritic cells, E2 binds to CD81 as well as BDCA-2 and DCIR, resulting in inhibition of IFN-alpha production and cell maturation (13, 14).

References

  1. Khan, A.G. et al. (2015) Curr. Opin. Virol. 12:53.
  2. Zona, L. et al. (2014) Viruses 6:875.
  3. Grakoui, A. et al. (1993) J. Virol. 67:1385.
  4. Op De Beeck, A. et al. (2004) J. Virol. 78:2994.
  5. Pileri, P. et al. (1998) Science 282:938.
  6. Scarselli, E. et al. (2002) EMBO J. 21:5017.
  7. Harris, H.J. et al. (2010) J. Biol. Chem. 285:21092.
  8. Falkowska, E. et al. (2007) J. Virol. 81:8072.
  9. Evans, M.J. et al. (2007) Nature 446:801.
  10. Ploss, A. et al. (2009) Nature 457:882.
  11. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
  12. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
  13. Tu, Z. et al. (2013) Cell. Immunol. 284:98.
  14. Florentin, J. et al. (2012) Blood 120:4544.

Long Name

Hepatitis C Virus 1b Envelope Glycoprotein E2

Alternate Names

HCV-E2, hcvGP-E2, Hep C E2

Entrez Gene IDs

951475 (Viral)

Gene Symbol

POLY

UniProt

NP_671491

Additional HCV E2 Products

Product Documents for Recombinant Viral HCV E2 Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Viral HCV E2 Protein, CF

For research use only

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