Recombinant Zebrafish Tie-2 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 928-T2
Key Product Details
Source
NS0
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Binding Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived zebrafish Tie-2 protein
| Zebrafish Tie-2 (Val22 - His741) Accession # O73791 |
DIEGRMD | Human IgG1 (Pro100 - Lys330) |
| N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val22
Predicted Molecular Mass
104.4 kDa (monomer)
SDS-PAGE
122 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized rzfTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can binds rhAngiopoietin-2 with a linear range of 2-100 ng/mL.
Immobilized rzfTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can binds rhAngiopoietin-2 with a linear range of 2-100 ng/mL.
Formulation, Preparation and Storage
928-T2
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Tie-2
Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis.
Zebrafish Tie-2 cDNA encodes a 1116 amino acid (aa) residue precursor protein shares 38% sequence homology with human Tie-2 in the extracellular domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind Tie-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-2 display similar angiogenesis defects.
References
- Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
- Takakura, N. et al. (1998) Immunity 9:677.
- Procopio, W. et al. (1999) J. Biol. Chem. 274:30196.
Long Name
Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 2
Alternate Names
CD202b, TEK, Tie2
Entrez Gene IDs
Gene Symbol
TEK
UniProt
Additional Tie-2 Products
Product Documents for Recombinant Zebrafish Tie-2 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Zebrafish Tie-2 Fc Chimera Protein, CF
For research use only
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