Recombinant Mouse SCF/c-kit Ligand Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35150
Key Product Details
Source
E. coli
Accession #
Conjugate
Unconjugated
Applications
Bioactivity, Flow Cytometry, SDS-PAGE
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 165 amino acids corresponding to SCF/c-kit Ligand Source: E. coli
Uniprot ID: P20826
Amino Acid Sequence: MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of SCF/c-kit Ligand as determined by LAL method.
Predicted Molecular Mass
18.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
SCF/c-kit Ligand protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferationassay using human TF-1 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.
Application Notes
Use in Flow reported in scientific literature (PMID:35874724).
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Mouse SCF/c-kit Ligand Protein
![SDS-PAGE: Recombinant Mouse SCF/c-kit Ligand Protein [NBP2-35150]](https://resources.bio-techne.com/images/products/Mouse-SCF-c-kit-Ligand-Protein-SDS-Page-NBP2-35150-img0001.jpg "SDS-PAGE: Recombinant Mouse SCF/c-kit Ligand Protein [NBP2-35150]")
SDS-PAGE: Recombinant Mouse SCF/c-kit Ligand Protein [NBP2-35150]
SDS-Page: Recombinant Mouse SCF/c-kit Ligand Protein [NBP2-35150] - SDS-PAGE of NBP2-35150Formulation, Preparation and Storage
NBP2-35150
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: SCF/c-kit Ligand
Long Name
Stem Cell Factor
Alternate Names
c-kit Ligand, DCUA, DFNA69, FPH2, FPHH, KITLG, KL-1, MGF, SHEP7, SLF
Gene Symbol
KITLG
Additional SCF/c-kit Ligand Products
Product Documents for Recombinant Mouse SCF/c-kit Ligand Protein
Product Specific Notices for Recombinant Mouse SCF/c-kit Ligand Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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