Recombinant Human Serum Amyloid A1 Protein

Name: Recombinant Human Serum Amyloid A1 Protein
Brand: Novus Biologicals
SKU: NBP2-34875

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34875

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Accession #

P0DJI8

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Serum Amyloid A1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 104 amino acids corresponding to Serum Amyloid A1 Source: E. coli

Uniprot ID: P0DJI8

Amino Acid Sequence: RSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISD ARENIQRFFG HGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY

Purity

> 98 % pure by SDS-PAGE and HPLC

Predicted Molecular Mass

11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human monocytes is in a concentration range of 10-100 ng/ml.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Serum Amyloid A1 Protein

SDS-PAGE: Recombinant Human Serum Amyloid A1 Protein [NBP2-34875]

SDS-Page: Recombinant Human Serum Amyloid A1 Protein [NBP2-34875] - man Serum Amyloid A1 Protein [NBP2-34875] - Human Serum Amyloid A1 Protein

Formulation, Preparation and Storage

NBP2-34875

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 9.0, 150 mM NaCl.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Serum Amyloid A1

Serum Amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein that circulates as part of the HDL complex. It can displace ApoA1 as the major apolipoprotein in HDL complexes and reduce the capacity of HDL for reverse cholesterol transport. In response to proinflammatory cytokines, SAA1 is prominently produced in liver and also in human but not mouse adipose tissue. Circulating SAA1 levels can increase by as much as 1000-fold during inflammation. SAA1 induces monocyte and macrophage chemotaxis and activation through binding to CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE. It can also opsonize and promote the clearance of Gram negative bacteria.

Alternate Names

PIG4, SAA1

Gene Symbol

SAA1

UniProt