Recombinant Rhesus Macaque Serum Amyloid A1 Protein

Name: Recombinant Rhesus Macaque Serum Amyloid A1 Protein
Brand: Novus Biologicals
SKU: NBP2-35320

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35320

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Serum Amyloid A1 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 104 amino acids corresponding to Serum Amyloid A1 Source: E. coli

Uniprot ID: F6V9N7

Amino Acid Sequence: RSWFSFLGEA YDGARDMWRA YSDMKEANYK NSDKYFHARG NYDAAQRGPG GVWAAEVISD ARENIQKLLG RGAEDTLADQ AANEWGRSGK DPNHFRPAGL PEKY

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of Serum Amyloid A1 as determined by LAL method.

Predicted Molecular Mass

11.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Serum Amyloid A1 protein is fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 10-100 ng/ml

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rhesus Macaque Serum Amyloid A1 Protein

SDS-PAGE: Recombinant Rhesus Macaque Serum Amyloid A1 Protein [NBP2-35320]

SDS-Page: Primate Serum Amyloid A1 Protein [NBP2-35320]

Formulation, Preparation and Storage

NBP2-35320

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Serum Amyloid A1

Serum Amyloid A protein-1 (SAA1) is a multifunctional apolipoprotein that circulates as part of the HDL complex. It can displace ApoA1 as the major apolipoprotein in HDL complexes and reduce the capacity of HDL for reverse cholesterol transport. In response to proinflammatory cytokines, SAA1 is prominently produced in liver and also in human but not mouse adipose tissue. Circulating SAA1 levels can increase by as much as 1000-fold during inflammation. SAA1 induces monocyte and macrophage chemotaxis and activation through binding to CD36/SR-B3, SR-B1, FPRL1, TLR2, and RAGE. It can also opsonize and promote the clearance of Gram negative bacteria.

Alternate Names

PIG4, SAA1

Gene Symbol

SAA1

Additional Serum Amyloid A1 Products

Product Documents for Recombinant Rhesus Macaque Serum Amyloid A1 Protein

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COA

Product Specific Notices for Recombinant Rhesus Macaque Serum Amyloid A1 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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