Recombinant Human SOD1/Cu-Zn SOD Protein

Name: Recombinant Human SOD1/Cu-Zn SOD Protein
Brand: Novus Biologicals
SKU: NBP2-34940

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34940

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P00441

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all SOD1/Cu-Zn SOD Proteins and Enzymes »

Product Specifications

Description

A homodimer, non-glycosylated polypeptide chain containing two 153 amino acid chains corresponding to SOD1/Cu-Zn SOD Source: E. coli

Uniprot ID: P00441

Amino Acid Sequence: ATKAVCVLKG DGPVQGIINF EQKESNGPVK VWGSIKGLTE GLHGFHVHEF GDNTAGCTSA GPHFNPLSRK HGGPKDEERH VGDLGNVTAD KDGVADVSIE DSVISLSGDH CIIGRTLVVH EKADDLGKGG NEESTKTGNA GSRLACGVIG IAQ

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of SOD1/Cu-Zn SOD as determined by LAL method.

Predicted Molecular Mass

31.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

SOD1/Cu-Zn SOD protein is fully biologically active when compared to standard. The potency per mg was determined by pyrogallol autoxidation method and was found to be more than 3000 U/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SOD1/Cu-Zn SOD Protein

SDS-PAGE: Recombinant Human SOD1/Cu-Zn SOD Protein [NBP2-34940]

SDS-Page: Human SOD1/Cu-Zn SOD Protein [NBP2-34940]

Formulation, Preparation and Storage

NBP2-34940

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: SOD1/Cu-Zn SOD

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -70 degrees C. Avoid repeated freeze/thaw cycles.

Long Name

Superoxide Dismutase-1

Alternate Names

Cu-Zn SOD, CuZn SOD, Ipo1, IPOA, SOD, cytosolic, SOD, Soluble

Gene Symbol

SOD1

UniProt