Recombinant Human SPARC Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61362
Key Product Details
Source
E. coli
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 286 amino acids corresponding to SPARC Source: E. coli
Uniprot ID: P09486
Amino Acid Sequence: APQQEALPDE TEVVEETVAE VTEVSVGANP VQVEVGEFDD GAEETEEEVV AENPCQNHHC KHGKVCELDE NNTPMCVCQD PTSCPAPIGE FEKVCSNDNK TFDSSCHFFA TKCTLEGTKK GHKLHLDYIG PCKYIPPCLD SELTEFPLRM RDWLKNVLVT LYERDEDNNL LTEKQKLRVK KIHENEKRLE AGDHPVELLA RDFEKNYNMY IFPVHWQFGQ LDQHPIDGYL SHTELAPLRA PLIPMEHCTT RFFETCDLDN DKYIALDEWA GCFGIKQKDI DKDLVI
Purity
>98%, by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of SPARC as determined by LAL method.
Predicted Molecular Mass
32.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
SPARC protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cell growth of Mv1Lu mink lung epithelial cells is less than 3.0 ug/mL, corresponding to a specific activity of > 333 IU/mg.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human SPARC Protein
SDS-Page: Recombinant Human SPARC Protein [NBP2-61362]
SDS-Page: Recombinant Human SPARC Protein [NBP2-61362]Formulation, Preparation and Storage
NBP2-61362
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: SPARC
Long Name
Secreted Protein Acidic and Rich in Cysteine
Alternate Names
BM-40, Osteonectin
Gene Symbol
SPARC
Additional SPARC Products
Product Documents for Recombinant Human SPARC Protein
Product Specific Notices for Recombinant Human SPARC Protein
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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