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Recombinant Human SPFH2 His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-23027

Novus Biologicals, part of Bio-Techne
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NBP2-23027

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 25-339 of Human SPFH2

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSKIEEGHI GVYYRGGALL TSTSGPGFHL MLPFITSYKS VQTTLQTDEV KNVPCGTSGG VMIYFDRIEV VNFLVPNAVY DIVKNYTADY DKALIFNKIH HELNQFCSVH TLQEVYIELF DQIDENLKLA LQQDLTSMAP GLVIQAVRVT KPNIPEAIRR NYELMESEKT KLLIAAQKQK VVEKEAETER KKALIEAEKV AQVAEITYGQ KVMEKETEKK ISEIEDAAFL AREKAKADAE CYTAMKIAEA NKLKLTPEYL QLMKYKAIAS NSKIYFGKDI PNMFMDSAGS VSKQFEGLAD KLSFGLEDEP LETATKEN

Purity

>95%, by SDS-PAGE

Predicted Molecular Mass

37.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human SPFH2 His Protein

SDS-PAGE: Recombinant Human SPFH2 His Protein [NBP2-23027]

SDS-PAGE: Recombinant Human SPFH2 His Protein [NBP2-23027]

SDS-Page: SPFH2 Protein [NBP2-23027]

Formulation, Preparation and Storage

NBP2-23027
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2M UREA, 20% glycerol
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPFH2

ERLIN2, also known as SPFH2, is SPFH domain-containing proteins that belong to the prohibitin family. Also, this protein is a ubiquitously expressed 339 amino acid protein that belongs to the band 7/mec-2 family. Localized to lipid raft-like domains in the membrane of the endoplasmic reticulum (ER), ERLIN2 plays a crucial role in the ER-associated degradation (ERAD) pathway that removes metabolically regulated and aberrant proteins from the ER. Recombinant human ERLIN2 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

C8orf2SPFH domain-containing protein 2, Endoplasmic reticulum lipid raft-associated protein 2, ER lipid raft associated 2, Erlin-2, MGC87072, NET32, SPFH domain family, member 2, SPFH2chromosome 8 open reading frame 2, Stomatin-prohibitin-flotillin-HflC/K domain-containing protein 2

Gene Symbol

ERLIN2

Additional SPFH2 Products

Product Documents for Recombinant Human SPFH2 His Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human SPFH2 His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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