Streptavidin Native Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29371

Novus Biologicals, part of Bio-Techne
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Key Product Details

Accession #

P22629

Conjugate

Alkaline Phosphatase

Applications

ELISA, Immunohistochemistry, Western Blot

View all Streptavidin Proteins and Enzymes »

Product Specifications

Description

Native Bacteria Streptavidin

Source: Electrophoretically pure Streptavidin (Accession #:P22629)

Store vial at 4C before opening. DO NOT FREEZE. Streptavidin Alkaline Phosphatase conjugated is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Purity

Immunoelectrophoresis (IEP)

Applications

ELISA (1:8000 - 1:32000)
Immunohistochemistry (1:200 - 1:1000)
Western Blot (1:1000 - 1:4000)

Application Notes

This product has been tested by dot blot and western blot and is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (NPP-10).

Protein / Peptide Type

Native Protein

Scientific Data Images for Streptavidin Native Protein

Western Blot: Streptavidin Native Protein [NBP2-29371]

Western Blot: Streptavidin Native Protein [NBP2-29371]

Western Blot: Streptavidin Native Protein [NBP2-29371] - Lane 1: Biotin conjugated Guinea Pig IgG Load: 50 ng per lane Secondary antibody: STREPTAVIDIN ALKALINE PHOSPHATASE Conjugated at 1:1,000 for 60 min at RT Block for30 min at RT Predicted/Observed Size: 28 and 55 kDa/28 and 55 kDa for Guinea Pig IgG.

Formulation, Preparation and Storage

NBP2-29371
Preparation Method This product was prepared from electrophoretically pure Streptavidin. Alkaline Phosphatase conjugated Streptavidin was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Streptavidin.
Formulation 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative 0.05% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C. Do not freeze.

Background: Streptavidin

Streptavidin is a a bacterial protein (from Streptomyces avidinii) that has an exceptionally high binding affinity for biotin (B7). Streptavidin-biotin binding is one of the strongest known non-covalent interactions and is highly resistant to many conditions that would typically cause dissociation (such as organic solvents, denaturants, detergents, and extreme temperatures or pH). Streptavidin's afinity for biotin can be employed in a variety of experimental uses, from purifications to standards, to means of detection or pull down experiments. Alkaline Phosphatase is an enzyme which removes phosphate groups from a variety of substate molecules. As the name implies, this enzyme functions best under basic pH. Alkaline Phosphatase can be utilized in molecular biology in DNA ligation experiments (keeping the DNA linear), radiolabeling preparations, and a detection mediator in ELISA experiments.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

UniProt

P22629 (Bacteria)

Additional Streptavidin Products

Product Documents for Streptavidin Native Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Streptavidin Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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