Recombinant Streptavidin His Protein

Name: Recombinant Streptavidin His Protein
Brand: Novus Biologicals
SKU: NBP2-22641

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22641

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Technical Data
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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Streptavidin Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 37-159 of Streptomyces Streptavidin

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAEAGI TGTWYNQLGS TFIVTAGADG ALTGTYESAV GNAESRYVLT GRYDSAPATD GSGTALGWTV AWKNNYRNAH SATTWSGQYV GGAEARINTQ WLLTSGTTEA NAWKSTLVGH DTFTKVKP

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

15.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Streptavidin His Protein

SDS-PAGE: Recombinant Streptavidin His Protein [NBP2-22641]

SDS-Page: Recombinant Bacterial Streptavidin Protein [NBP2-22641]

Formulation, Preparation and Storage

NBP2-22641

Formulation	20 mM Tris-HCl buffer (pH 8.0), 0.15 M NaCl, 20% glycerol
Preservative	No Preservative
Concentration	0.25 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Streptavidin

Streptavidin, a tetrameric protein secreted by Streptomyces avidinii, binds tightly to a small growth factor biotin. It finds wide use in molecular biology through its extraordinarily strong affinity for the vitamin biotin; the dissociation constant (Kd) of the biotin-streptavidin complex is on the order of ~10-15 mol/L. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. Recombinant Streptomyces streptavidin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

SA V1; SA V2; Streptavidin V1; Streptavidin V2

Additional Streptavidin Products

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COA

Product Specific Notices for Recombinant Streptavidin His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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