Recombinant Human TARS His Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-99100

Novus Biologicals, part of Bio-Techne
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NBP1-99100-50ug
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Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TARS Proteins and Enzymes »

Product Specifications

Description

A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-723 of Human TARS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MFEEKASSPS GKMGGEEKPI GAGEEKQKEG GKKKNKEGSG DGGRAELNPW PEYIYTRLEM YNILKAEHDS ILAEKAEKDS KPIKVTLPDG KQVDAESWKT TPYQIACGIS QGLADNTVIA KVNNVVWDLD RPLEEDCTLE LLKFEDEEAQ AVYWHSSAHI MGEAMERVYG GCLCYGPPIE NGFYYDMYLE EGGVSSNDFS SLEALCKKII KEKQAFERLE VKKETLLAMF KYNKFKCRIL NEKVNTPTTT VYRCGPLIDL CRGPHVRHTG KIKALKIHKN SSTYWEGKAD METLQRIYGI SFPDPKMLKE WEKFQEEAKN RDHRKIGRDQ ELYFFHELSP GSCFFLPKGA YIYNALIEFI RSEYRKRGFQ EVVTPNIFNS RLWMTSGHWQ HYSENMFSFE VEKELFALKP MNCPGHCLMF DHRPRSWREL PLRLADFGVL HRNELSGALT GLTRVRRFQQ DDAHIFCAME QIEDEIKGCL DFLRTVYSVF GFSFKLNLST RPEKFLGDIE VWDQAEKQLE NSLNEFGEKW ELNSGDGAFY GPKIDIQIKD AIGRYHQCAT IQLDFQLPIR FNLTYVSHDG DDKKRPVIVH RAILGSVERM IAILTENYGG KWPFWLSPRQ VMVVPVGPTC DEYAQKVRQQ FHDAKFMADI DLDPGCTLNK KIRNAQLAQY NFILVVGEKE KISGTVNIRT RDNKVHGERT ISETIERLQQ LKEFRSKQAE EEF

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

85.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TARS His Protein

SDS-PAGE: Recombinant Human TARS His Protein [NBP1-99100]

SDS-PAGE: Recombinant Human TARS His Protein [NBP1-99100]

SDS-Page: Recombinant Human TARS His Protein [NBP1-99100] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Formulation, Preparation and Storage

NBP1-99100
Formulation 20 mM Tris-HCl buffer (pH 8.0), 2 mM DTT, 20% glycerol, 150 mM NaCl
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TARS

TARS (Threonyl-tRNA synthetase, cytoplasmic) belongs to the class-II aminoacyl-tRNA synthetase family. This protein has its main role in tRNA aminoacylation. The N-terminal domain of the enzyme is responsible for the competition with the ribosome whilst the catalytic and the C-terminal domain are involved in binding the two anticodon arm-like structures in the operator. Recombinant human TARS protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Alternate Names

cytoplasmic, EC 6.1.1.3, MGC9344, Threonine--tRNA ligase, threonyl-tRNA synthetase, threonyl-tRNA synthetase, cytoplasmic

Gene Symbol

TARS1

Additional TARS Products

Product Documents for Recombinant Human TARS His Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human TARS His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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