Can I do the function test using this protein?

Unfortunately, we have not yet tested NBP2-22850 for functionality; therefore, we are unable to guarantee its performance in a functional assay.

Do you remove endotoxin from your recombinant human TDP-43 (Catalog # NBP2-22850)?

We do not prepare this protein for removal of the endotoxin other than the purification that is done standardly. This protein is made only as a control for PAGE/WESTERN where the endotoxin would not matter rather than as an active protein. We have not measured activity at all and would not be able to say if the protein has any activity.

Recombinant Human TDP-43/TARDBP His Protein

Name: Recombinant Human TDP-43/TARDBP His Protein
Brand: Novus Biologicals
SKU: NBP2-22850

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22850

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all TDP-43/TARDBP Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-414 of Human TDP-43/TARDBP

Source: E.coli

Amino Acid Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSEY IRVTEDENDE PIEIPSEDDG TVLLSTVTAQ FPGACGLRYR NPVSQCMRGV RLVEGILHAP DAGWGNLVYV VNYPKDNKRK MDETDASSAV KVKRAVQKTS DLIVLGLPWK TTEQDLKEYF STFGEVLMVQ VKKDLKTGHS KGFGFVRFTE YETQVKVMSQ RHMIDGRWCD CKLPNSKQSQ DEPLRSRKVF VGRCTEDMTE DELREFFSQY GDVMDVFIPK PFRAFAFVTF ADDQIAQSLC GEDLIIKGIS VHISNAEPKH NSNRQLERSG RFGGNPGGFG NQGGFGNSRG GGAGLGNNQG SNMGGGMNFG AFSINPAMMA AAQAALQSSW GMMGMLASQQ NQSGPSGNNQ NQGNMQREPN QAFGSGNNSY SGSNSGAAIG WGSASNAGSG SGFNGGFGSS MDSKSSGWGM

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

48.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TDP-43/TARDBP His Protein

SDS-PAGE: Recombinant Human TDP-43/TARDBP His Protein [NBP2-22850]

SDS-Page: Recombinant Human TDP-43/TARDBP Protein [NBP2-22850]

Formulation, Preparation and Storage

NBP2-22850

Formulation	20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TDP-43/TARDBP

TARDBP, as known as TAR DNA binding protein, was originally identified as a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. It has also been identified in individuals diagnosed with chronic traumatic encephalopathy, a condition that often mimics ALS and that has been associated with athletes who have experienced multiple concussions and other types of head injury. It has been shown to bind both DNA and RNA and have multiple functions in transcriptional repression, pre-mRNA splicing and translational regulation. Recombinant human TARDBP protein protein, fused to His-tag at N-terminus, was expressed in E.coli.

Long Name

TAR DNA-binding Protein 43

Alternate Names

ALS10, TARDBP, TDP43

Gene Symbol

TARDBP

Additional TDP-43/TARDBP Products

Product Documents for Recombinant Human TDP-43/TARDBP His Protein

Product Datasheets

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COA

Product Specific Notices for Recombinant Human TDP-43/TARDBP His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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