Recombinant Human TFF2 Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35058
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 106 amino acids corresponding to TFF2, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds Source: E. coli
Uniprot ID: Q03403
Amino Acid Sequence: EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 1 EU/ug of TFF2 as determined by LAL method.
Predicted Molecular Mass
12.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
Spasmolytic Polypeptide protein is fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 x 10^5 IU/mg.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35058
| Formulation | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: TFF2
Long Name
Trefoil Factor 2
Alternate Names
SML1, SP, Spasmolysin, Spasmolytic Polypeptide, TFF2
Gene Symbol
TFF2
UniProt
Additional TFF2 Products
Product Documents for Recombinant Human TFF2 Protein
Product Specific Notices for Recombinant Human TFF2 Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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