Recombinant Mouse Thrombopoietin/THPO Protein

Name: Recombinant Mouse Thrombopoietin/THPO Protein
Brand: Novus Biologicals
SKU: NBP2-35186

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35186

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P40226

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all Thrombopoietin/Tpo Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 174 amino acids corresponding to Thrombopoietin/THPO Source: E. coli

Uniprot ID: P40226

Amino Acid Sequence: SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Thrombopoietin/THPO as determined by LAL method.

Predicted Molecular Mass

18.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Thrombopoietin Protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human MO7e cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Mouse Thrombopoietin/THPO Protein

SDS-PAGE: Recombinant Mouse Thrombopoietin/THPO Protein [NBP2-35186]

SDS-Page: Recombinant Mouse Thrombopoietin/THPO Protein [NBP2-35186]

Formulation, Preparation and Storage

NBP2-35186

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 5% Trehalose.
Preservative	5% Trehalose
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: Thrombopoietin/Tpo

Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders. Tpo additionally promotes platelet aggregation, ECM adhesion, and activation. In the brain, Tpo promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling.

Alternate Names

MGDF, MK-CSF, MKCSF, MPLLG, THCYT1, THPO, Tpo

Gene Symbol

THPO

UniProt