Recombinant Mouse TNF RI/TNFRSF1A Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35181
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 191 amino acids corresponding to TNF RI/TNFRSF1A Source: E. coli
Uniprot ID: P25118
Amino Acid Sequence: IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of TNF RI/TNFRSF1A as determined by LAL method.
Predicted Molecular Mass
21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
TNF Receptor I Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 1 ug/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 0.1 ng/mL of rMuTNF-alpha.
Protein / Peptide Type
Recombinant Protein
Formulation, Preparation and Storage
NBP2-35181
| Formulation | Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: TNF RI/TNFRSF1A
Long Name
Tumor Necrosis Factor Receptor I
Alternate Names
CD120a, TNFRI, TNFRSF1A, sTNF RI, sTNF RI/TNFRSF1A
Gene Symbol
TNFRSF1A
UniProt
Additional TNF RI/TNFRSF1A Products
Product Documents for Recombinant Mouse TNF RI/TNFRSF1A Protein
Product Specific Notices for Recombinant Mouse TNF RI/TNFRSF1A Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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