Recombinant Mouse TNF RI/TNFRSF1A Protein

Name: Recombinant Mouse TNF RI/TNFRSF1A Protein
Brand: Novus Biologicals
SKU: NBP2-35181

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35181

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Product Datasheet / COA

Catalog #

Availability

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Product Specifications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P25118

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TNF RI/TNFRSF1A Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 191 amino acids corresponding to TNF RI/TNFRSF1A Source: E. coli

Uniprot ID: P25118

Amino Acid Sequence: IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of TNF RI/TNFRSF1A as determined by LAL method.

Predicted Molecular Mass

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TNF Receptor I Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 1 ug/ml, corresponding to a specific activity of > 1000 IU/mg in the presence of 0.1 ng/mL of rMuTNF-alpha.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35181

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TNF RI/TNFRSF1A

TNF RI, also named as TNFR-I, TNF R1, TNFRSF1A, p55 and p60, belongs to the tumor necrosis factor receptor superfamily and it is encoded by the TNFRSF1A gene in human. TNF RI is a universally expressed protein and is thought to form homotrimers after ligand binding. TNF RI is one of the major receptors for the TNF-alpha. This receptor can activate the transcription factor NF-kappa B, mediate apoptosis, and function as a regulator of inflammation. Murine TNF RI shares 70 %, 88 %, 67 %, 70 % and 64 % a.a. sequence identity with human, rat, canine, feline and porcine TNF RI, respectively.

Long Name

Tumor Necrosis Factor Receptor I

Alternate Names

CD120a, TNFRI, TNFRSF1A, sTNF RI, sTNF RI/TNFRSF1A

Gene Symbol

TNFRSF1A

UniProt