Recombinant Human TNF RII/TNFRSF1B Protein

Name: Recombinant Human TNF RII/TNFRSF1B Protein
Brand: Novus Biologicals
SKU: NBP2-35275

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35275

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P20333

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TNF RII/TNFRSF1B Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 184 amino acids corresponding to TNF RII/TNFRSF1B Source: E. coli

Uniprot ID: P20333

Amino Acid Sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of TNF RII/TNFRSF1B as determined by LAL method.

Predicted Molecular Mass

20.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TNF Receptor II Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.2 ug/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TNF RII/TNFRSF1B Protein

SDS-PAGE: Recombinant Human TNF RII/TNFRSF1B Protein [NBP2-35275]

SDS-Page: Human TNF RII/TNFRSF1B Protein [NBP2-35275]

Formulation, Preparation and Storage

NBP2-35275

Formulation	Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TNF RII/TNFRSF1B

Tumor Necrosis Factor Receptor-I (TNF RI, also known as p55/p60) and TNF RII (also known as p75/p80) are both high affinity receptors for TNF-alpha and -beta, potent mediators of multiple aspects of inflammatory immune responses. Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Whereas all cell types are thought to express TNF RI, TNF RII expression is limited primarily to hematopoietic cells and cells of the immune system. Most of the biological functions of TNF are mediated via TNF RI. Soluble forms of both TNF RI and TNF RII, generated as a result of proteolytic cleavage of the extracellular domains, have been detected in vivo in various biological fluids. The soluble TNF receptors can bind TNF with high affinity and functions as TNF antagonists.

Long Name

Tumor Necrosis Factor Receptor II

Alternate Names

CD120b, TNFRII, TNFRSF1B, sTNF RI, sTNF RI/TNFRSF1A

Gene Symbol

TNFRSF1B

UniProt