Recombinant Human TNF RII/TNFRSF1B Protein
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35275
Key Product Details
Product Specifications
Description
A single non-glycosylated polypeptide chain containing 184 amino acids corresponding to TNF RII/TNFRSF1B Source: E. coli
Uniprot ID: P20333
Amino Acid Sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Level
Less than 0.1 EU/ug of TNF RII/TNFRSF1B as determined by LAL method.
Predicted Molecular Mass
20.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Activity
TNF Receptor II Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.2 ug/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.
Protein / Peptide Type
Recombinant Protein
Scientific Data Images for Recombinant Human TNF RII/TNFRSF1B Protein
![SDS-PAGE: Recombinant Human TNF RII/TNFRSF1B Protein [NBP2-35275]](https://resources.bio-techne.com/images/products/Human-TNF-RII-TNFRSF1B-Protein-SDS-Page-NBP2-35275-img0001.jpg "SDS-PAGE: Recombinant Human TNF RII/TNFRSF1B Protein [NBP2-35275]")
SDS-PAGE: Recombinant Human TNF RII/TNFRSF1B Protein [NBP2-35275]
SDS-Page: Human TNF RII/TNFRSF1B Protein [NBP2-35275]Formulation, Preparation and Storage
NBP2-35275
| Formulation | Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4. |
| Preservative | No Preservative |
| Concentration | Lyoph |
| Reconstitution | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
| Format | Carrier-Free |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Background: TNF RII/TNFRSF1B
Long Name
Tumor Necrosis Factor Receptor II
Alternate Names
CD120b, TNFRII, TNFRSF1B, sTNF RI, sTNF RI/TNFRSF1A
Gene Symbol
TNFRSF1B
UniProt
Additional TNF RII/TNFRSF1B Products
Product Documents for Recombinant Human TNF RII/TNFRSF1B Protein
Product Specific Notices for Recombinant Human TNF RII/TNFRSF1B Protein
This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.
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