Recombinant Mouse TRAIL/TNFSF10 Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61331

Novus Biologicals, part of Bio-Techne
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NBP2-61331-1000ug
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NBP2-61331-10ug
NBP2-61331-500ug
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Key Product Details

Source

E. coli

Conjugate

Unconjugated

Applications

Bioactivity

View all TRAIL/TNFSF10 Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 175 amino acids corresponding to TRAIL/TNFSF10 Source: E. coli

Uniprot ID: P50592

Amino Acid Sequence: MPRGGRPQKV AAHITGITRR SNSALIPISK DGKTLGQKIE SWESSRKGHS FLNHVLFRNG ELVIEQEGLY YIYSQTYFRF QEAEDASKMV SKDKVRTKQL VQYIYKYTSY PDPIVLMKSA RNSCWSRDAE YGLYSIYQGG LFELKKNDRI FVSVTNEHLM DLDQEASFFG AFLIN

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of TRAIL/TNFSF10 as determined by LAL method.

Predicted Molecular Mass

20.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TRAIL/TNFSF10 protein is fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 x 10^6 IU/mg in the presence of actinomycin D.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-61331
Formulation Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4, with 3 mM DTT.
Preservative No Preservative
Concentration Lyoph
Reconstitution Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL. Apportion stock solutions into working aliquots and store at <-20C.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TRAIL/TNFSF10

Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues.

Long Name

TNF-related Apoptosis-inducing Ligand

Alternate Names

CD253, TNFSF10

Gene Symbol

TNFSF10

Additional TRAIL/TNFSF10 Products

Product Documents for Recombinant Mouse TRAIL/TNFSF10 Protein

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse TRAIL/TNFSF10 Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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