Recombinant Human TRAILR2/TNFRSF10B Protein

Name: Recombinant Human TRAILR2/TNFRSF10B Protein
Brand: Novus Biologicals
SKU: NBP2-35030

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35030

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Product Datasheet / COA

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

O14763

Conjugate

Unconjugated

Applications

Bioactivity, SDS-PAGE

View all TRAIL R2/TNFRSF10B Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 132 amino acids corresponding to TRAILR2/TNFRSF10B Source: E. coli

Uniprot ID: O14763

Amino Acid Sequence: ESALITQQDL APQQRAAPQQ KRSSPSEGLC PPGHHISEDG RDCISCKYGQ DYSTHWNDLL FCLRCTRCDS GEVELSPCTT TRNTVCQCEE GTFREEDSPE MCRKCRTGCP RGMVKVGDCT PWSDIECVHK ES

Purity

> 97 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of TRAILR2/TNFRSF10B as determined by LAL method.

Predicted Molecular Mass

14.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

TRAIL-R2 protein is fully biologically active when compared to standard. rHusTRAIL-R2 reduced the production of LPS-induced TNF by its ability to neutralize endogenous TRAIL in fresh human PBMC. In this assay, endogenous TRAIL is induced during a 24 hour exposure to LPS (10 ng/mL) but in the presence of rHusTRAIL-R2, TRAIL-induced TNF is suppressed.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human TRAILR2/TNFRSF10B Protein

SDS-PAGE: Recombinant Human TRAILR2/TNFRSF10B Protein [NBP2-35030]

SDS-Page: Human TRAIL R2/TNFRSF10B Protein [NBP2-35030]

Formulation, Preparation and Storage

NBP2-35030

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Background: TRAIL R2/TNFRSF10B

Tumor necrosis factor-related apoptosis-inducing ligand Receptor 2 (TRAIL-R2) is a cell-surface receptor involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced cell-death signaling. The death ligand TRAIL bears high potential as a new anticancer agent, as binding to the death receptors TRAIL-R1 or TRAIL-R2 triggers apoptosis in most cancer cells. TRAIL-R2 has been shown to be associated with a decrease in the survival rates of breast cancer patients.

Long Name

TRAIL Receptor 2

Alternate Names

CD262, DR5, TNFRSF10B, TRAILR2, sTRAILR2, sTRAILR2/TNFRSF10B, TRAILR2/TNFRSF10B

Gene Symbol

TNFRSF10B

UniProt