Recombinant Human Tryptase beta-2/TPSB2 His Protein

Name: Recombinant Human Tryptase beta-2/TPSB2 His Protein
Brand: Novus Biologicals
SKU: NBP2-22894

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22894

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Technical Data
Citations & Reviews

Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Accession #

P20231

Tag

His

Conjugate

Unconjugated

Applications

SDS-PAGE

View all Tryptase beta-2/TPSB2 Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 31-275 of Human Tryptase alpha/TPS1

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMIVGGQ EAPRSKWPWQ VSLRVHGPYW MHFCGGSLIH PQWVLTAAHC VGPDVKDLAA LRVQLREQHL YYQDQLLPVS RIIVHPQFYT AQIGADIALL ELEEPVNVSS HVHTVTLPPA SETFPPGMPC WVTGWGDVDN DERLPPPFPL KQVKVPIMEN HICDAKYHLG AYTGDDVRIV RDDMLCAGNT RRDSCQGDSG GPLVCKVNGT WLQAGVVSWG EGCAQPNRPG IYTRVTYYLD WIHHYVPKKP

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

30.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Specificity

Due to sequence identity this antibody will likely cross react with tryptase alpha/beta 1.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Tryptase beta-2/TPSB2 His Protein

SDS-PAGE: Recombinant Human Tryptase beta-2/TPSB2 His Protein [NBP2-22894]

SDS-Page: Recombinant Human Tryptase alpha/TPS1 Protein [NBP2-22894]

Formulation, Preparation and Storage

NBP2-22894

Formulation	20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.4M UREA
Preservative	No Preservative
Concentration	1 mg/ml
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Tryptase beta-2/TPSB2

Mast Cell Tryptase Protein, also known as tryptase alpha/beta-1, is a tryptase that is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It is enzymatically active only as a heparin-stabilized tetramer, and it is resistant to all known an endogenous proteinase inhibitor. This protein has been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. Recombinant human Mast Cell Tryptase Protein, fused to His-tag at N-terminus, was expressed in E.coli.

Alternate Names

EC 3.4.21, EC 3.4.21.59, mast cell tryptase beta II, mast cell tryptase beta III, TPS2, TPS2tryptase beta-2, TPSB2, tryptase beta 2, tryptase beta 2 (gene/pseudogene), tryptase beta II, tryptase beta III, Tryptase beta2, Tryptase beta-2, Tryptase II, tryptase III, Tryptase-2, tryptaseB, tryptaseC, Tryptase-C

Gene Symbol

TPSB2

UniProt