Recombinant Human Tyrosine Hydroxylase His Protein

Name: Recombinant Human Tyrosine Hydroxylase His Protein
Brand: Novus Biologicals
SKU: NBP1-42461

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42461

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Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Source

E. coli

Tag

His

Conjugate

Unconjugated

Applications

Immunohistochemistry, SDS-PAGE, Western Blot

View all Tyrosine Hydroxylase Proteins and Enzymes »

Product Specifications

Description

Recombinant Tyrosine Hydroxylase protein with an N-terminal His tag and corresponding to amino acids 1 - 497.

Source: E. coli

(Accession #: P07101-2)

Purity

>90%, by SDS-PAGE

Predicted Molecular Mass

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

This Tyrosine Hydroxylase protein is useful as a positive control for Western blotting. This protein has been tested using NB300-109 for detection on a Western blot. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24092694).

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human Tyrosine Hydroxylase His Protein

Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461]

Western Blot: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - Novus Biologicals antibody NB300-109 was used to detect the protein on a Western blot. 150 ng of protein were added to the Western blot.

SDS-PAGE: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461]

SDS-Page: Recombinant Human Tyrosine Hydroxylase His Protein [NBP1-42461] - This product has a purity >95%. This protein was run on a 4-20% SDS-PAGE gel with 5 ug protein loaded.

Formulation, Preparation and Storage

NBP1-42461

Formulation	300 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.47 mM KH2PO4, 5% glycerol, 1 mM DTT, pH 7.4
Preservative	N/A
Concentration	0.5 mg/ml
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -80C. Avoid freeze-thaw cycles.

Background: Tyrosine Hydroxylase

The tetrameric enzyme tyrosine hydroxylase (TH), also designated as tyrosine 3-monooxygenase (TY3H), is the rate-limiting enzyme for catecholamine biosynthesis and has a theoretical molecular weight of 60 kDa. Encoded by the TH gene, TH catalyzes the conversion of the amino acid L-tyrosine to L-3,4- dihydroxyphenylalanine (L-dopa) in the central nervous system and adrenal medulla. The precursor of catecholamines, L-dopa, is converted to dopamine for use in the biosynthesis of neurotransmitters norepinephrine (noradrenaline) and epinephrine (adrenaline) (1) . Dysfunction in the TH gene, and thus the enzymatic activity by TH, dysregulates catecholamine synthesis.

Two transcription factor binding sites in the proximal region of the TH gene, the TPA-responsive element (TRE) and the c-AMP responsive element (CRE), have been implicated in the complex regulation of the TH gene. Dysregulation of breakdown for the amino acid, tyrosine, by TH is a result of a genetic disorder that results in Tyrosinemia (high levels of tyrosine in the blood, tissue and organs).

Tyrosine hydroxylase deficiency is a disorder that primarily affects movement, where individuals display symptoms that include lack of coordination when walking, postural tremors and unusual body positioning. TH deficient dopamine-responsive dystonia (DRD), also known as Segawa syndrome, is a rare genetic disorder that is associated with low levels of TH and is diagnosed during childhood with characteristic symptoms including increased muscle tone (dystonia) and signs of Parkinsonism like bradykinesia, tremors, rigidity and postural instability (2). Correspondingly, TH is also linked to Parkinson's disease in older adults, where low dopamine levels are a consistent neurochemical abnormality. Functional polymorphisms of the TH gene may be involved in the pathogenesis of neuropsychiatric diseases such as schizophrenia and other affective disorders where dopamine is often dysregulated (3).

References

1. Hamanaka, Y., & Mizunami, M. (2019). Tyrosine hydroxylase-immunoreactive neurons in the mushroom body of the field cricket, Gryllus bimaculatus. Cell Tissue Res, 376(1), 97-111. doi:10.1007/s00441-018-2969-9

2. Li, L., & Zhou, F. M. (2013). Parallel dopamine D1 receptor activity dependence of l-Dopa-induced normal movement and dyskinesia in mice. Neuroscience, 236, 66-76. doi:10.1016/j.neuroscience.2012.12.065

3. Borkar, C. D., Bharne, A. P., Nagalakshmi, B., Sakharkar, A. J., Subhedar, N. K., & Kokare, D. M. (2018). Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms. Neuroscience, 375, 94-107. doi:10.1016/j.neuroscience.2018.01.056

Alternate Names

TH, TYH

Gene Symbol

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COA

Product Specific Notices for Recombinant Human Tyrosine Hydroxylase His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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