Recombinant Human UbcH10/UBE2C His (N-Term) Protein

Name: Recombinant Human UbcH10/UBE2C His (N-Term) Protein
Brand: Novus Biologicals
SKU: NBP2-35090

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35090

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Product Specifications
Scientific Data
Preparation & Storage
Background
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Specific Notices

Key Product Details

Source

E. coli

Tag

His (N-Term)

Conjugate

Unconjugated

Applications

SDS-PAGE

View all UbcH10/UBE2C Proteins and Enzymes »

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 179 amino acids corresponding to UbcH10/UBE2C and 12 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

Amino Acid Sequence: MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of UbcH10/UBE2C as determined by LAL method.

Predicted Molecular Mass

21.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human UbcH10/UBE2C His (N-Term) Protein

SDS-PAGE: Recombinant Human UbcH10/UBE2C His (N-Term) Protein [NBP2-35090]

SDS-Page: Human UbcH10/UBE2C His (N-Term) Protein [NBP2-35090]

Formulation, Preparation and Storage

NBP2-35090

Formulation	A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 8.0, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative	5% Trehalose
Concentration	Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution	Please contact technical support for detailed reconstitution instructions.
Format	Carrier-Free
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH10/UBE2C

Human Ubquitin Conjugating Enzyme E2 C (UBE2C)/UBCH10 is an essential mediator of mitotic destruction events and cell cycle progression. It catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis This activity is essential at then end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions.

Alternate Names

UBE2C

Gene Symbol