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Recombinant Human UbcH5c/UBE2D3 His (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35092

Novus Biologicals, part of Bio-Techne
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NBP2-35092-100ug
NBP2-35092-10ug
NBP2-35092-1mg
NBP2-35092-500ug

Key Product Details

Source

E. coli

Accession #

Tag

His (N-Term)

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 147 amino acids corresponding to UbcH5c/UBE2D3 and 8 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

Uniprot ID: P61077

Amino Acid Sequence: MHHHHHHAMA LKRINKELSD LARDPPAQCS AGPVGDDMFH WQATIMGPND SPYQGGVFFL TIHFPTDYPF KPPKVAFTTR IYHPNINSNG SICLDILRSQ WSPALTISKV LLSICSLLCD PNPDDPLVPE IARIYKTDRD KYNRISREWT QKYAM

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of UbcH5c/UBE2D3, His as determined by LAL method.

Predicted Molecular Mass

17.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35092
Formulation A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 6.5, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative 5% Trehalose
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Reconstitution Please contact technical support for detailed reconstitution instructions.
Format Carrier-Free
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH5c/UBE2D3

Ubiquitin Conjugating Enzyme E2 D3 (UBE2D3)/UBC5C enzymes are human homologs of the yeast UBC4/5 family and play many important regulatory roles in inflammation and cancer. UbcH5a mediates the degradation of a myriad of short-lived regulatory proteins (such as p53 in the presence of E6/E6-AP or MDM2, c-Fos, I kappa B alpha, p105) and abnormal proteins. UBE2D3 has 88% and 89% sequence identity with UbcH5a and UbcH5b respectively.

Alternate Names

UBC5C, UBE2D3

Gene Symbol

UBE2D3

UniProt

Additional UbcH5c/UBE2D3 Products

Product Documents for Recombinant Human UbcH5c/UBE2D3 His (N-Term) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human UbcH5c/UBE2D3 His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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