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Recombinant Human UbcH7/UBE2L3 His (N-Term) Protein

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-35110

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-35110-100ug
NBP2-35110-10ug
NBP2-35110-1mg
NBP2-35110-500ug

Key Product Details

Source

E. coli

Tag

His (N-Term)

Conjugate

Unconjugated

Applications

SDS-PAGE

Product Specifications

Description

A single non-glycosylated polypeptide chain containing 154 amino acids corresponding to UbcH7/UBE2L3 and 8 amino acid vector sequence including 6x His tag at N-terminus Source: E. coli

Amino Acid Sequence: MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD

Purity

>95%, by SDS-PAGE and HPLC

Endotoxin Level

Less than 1 EU/ug of UbcH7/UBE2L3 as determined by LAL method.

Predicted Molecular Mass

18.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Protein / Peptide Type

Recombinant Protein

Formulation, Preparation and Storage

NBP2-35110
Formulation A 0.2 um filtered concentrated solution in 50 mM HEPES, pH 7.0, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
Preservative 5% Trehalose
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Format Carrier-Free
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at -20C. Avoid freeze-thaw cycles.

Background: UbcH7/UBE2L3

Human Ubquitin Conjugating Enzyme E2 L3 (UBE2L3)/UBCH7 is a class I enzyme which functions in the stress response and the control of transcription factors. The enzyme is ubiquitously expressed with high levels of expression seen in adult muscle. UBCH7 mediates the selective degradation of short-lived and abnormal proteins and is highly homologous to UBCH5. It has been demonstrated to participate in the ubiquitinylation of p53, c-Fos and NF-kappaB. UBCH7 is one of two E2s (UBCH5 being the other) with which HECT domain proteins interact with UBCH7 being able to efficiently substitute for UBCH5 in E6-APdependent ubiquitinylation.

Alternate Names

E2-F1, L-UBC, UBCE7, UbcM4, UBE2L3

Gene Symbol

UBE2L3

Additional UbcH7/UBE2L3 Products

Product Documents for Recombinant Human UbcH7/UBE2L3 His (N-Term) Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Recombinant Human UbcH7/UBE2L3 His (N-Term) Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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